BCAP31 Antibody

Western Blot: BCAP31 Antibody [NB100-1016] - Staining of Human Breast lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: BCAP31 Antibody [NB100-1016] - Staining of paraffin embedded Human Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: BCAP31 Antibody [NB100-1016] - Staining of paraffin embedded Human Cerebellum. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: BCAP31 Antibody [NB100-1016] - Staining of paraffin embedded Human Kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BCAP31 Antibody Summary

• Description: Novus Biologicals Goat BCAP31 Antibody (NB100-1016) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-BCAP31 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QAAVDGPMDKKEE corresponding to C-Terminus according to NP_001132929.1, NP_005736.3.
• Localization: Endoplasmic reticulum and Golgi Apparatus
• Specificity: This antibody is expected to recognise isoform a (NP_001132929.1) and isoform b (NP_005736.3; NP_001132913.1).
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BCAP31
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/mL
• Control:

  BCAP31 Overexpression Lysate
  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Placenta Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BCAP31 Antibody

• 6C6-Ag
• BAP31
• Bap31,6C6-AG
• BAP31DXS1357ECDM
• BCAP31
• B-cell receptor-associated protein 31
• BCR-associated protein 31
• BCR-associated protein Bap31,6C6-AG tumor-associated antigen
• p28 Bap31,6C6-Ag
• p28
• Protein CDM

Background

BCAP31 may play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAP31 Antibody (NB100-1016)(1)

Publication using NB100-1016

• Adachi T, Schamel WW, Kim KM et al. The specificity of association of the IgD molecule with the accessory proteinsBAP31/BAP29 lies in the IgD transmembrane sequence. EMBO J 1996-04-01 [PMID: 8612576]

Bioinformatics

• Gene Symbol: BCAP31
• Entrez:
  • 10134(Human)
• Uniprot:
  • NP_005736.(Human)