BCAP31 Antibody

Images

 
Western Blot: BCAP31 Antibody [NB100-1016] - (0.1ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.chemiluminescence.
Immunohistochemistry-Paraffin: BCAP31 Antibody [NB100-1016] - staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: BCAP31 Antibody [NB100-1016] - staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BCAP31 Antibody Summary

Immunogen
Peptide with sequence C-QAAVDGPMDKKEE corresponding to C-Terminus according to NP_001132929.1, NP_005736.3.
Localization
Endoplasmic reticulum and Golgi Apparatus
Specificity
This antibody is expected to recognise isoform a (NP_001132929.1) and isoform b (NP_005736.3; NP_001132913.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BCAP31
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 UG/ML
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 30 kDa band observed in human placenta and brain lysate (predicted MW of 28 kDa band according to NP_005736).
IHC: Paraffin embedded Human Kidney and Brain (Cerebellum).
Positive Control
BCAP31 Lysate (NBL1-07934)
Brain Lysate (NB820-59177)
Placenta Lysate (NB820-59248)
Publications
Read Publication using NB100-1016.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCAP31 Antibody

  • 6C6-Ag
  • BAP31
  • Bap31,6C6-AG
  • BAP31DXS1357ECDM
  • BCAP31
  • B-cell receptor-associated protein 31
  • BCR-associated protein 31
  • BCR-associated protein Bap31,6C6-AG tumor-associated antigen
  • p28 Bap31,6C6-Ag
  • p28
  • Protein CDM

Background

BCAP31 may play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BCAP31 Antibody (NB100-1016)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BCAP31
Entrez
Uniprot