BATF Antibody (HL1870) - Azide and BSA Free

Western Blot: BATF Antibody (HL1870) - Azide and BSA Free [NBP3-25377] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with BATF antibody [HL1870] (NBP3-25377) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: BATF Antibody (HL1870) - Azide and BSA Free [NBP3-25377] - BATF antibody [HL1870] detects BATF protein by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BATF stained by BATF antibody [HL1870] (NBP3-25377) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.

Western Blot: BATF Antibody (HL1870) - Azide and BSA Free [NBP3-25377] - MCF-7 whole cell and nuclear extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with BATF antibody [HL1870] (NBP3-25377) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Rt, Po, Bv, RM
• Applications: WB, ICC/IF
• Clone: HL1870
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BATF Antibody (HL1870) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit BATF Antibody (HL1870) - Azide and BSA Free (NBP3-25377) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human BATF
• Source: HEK293
• Predicted Species: Rat (98%), Porcine (96%), Bovine (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BATF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for BATF Antibody (HL1870) - Azide and BSA Free

• basic leucine zipper transcription factor, ATF-like
• BATF
• BATF1basic leucine zipper transcriptional factor ATF-like
• B-ATFSFA2
• B-cell-activating transcription factor
• SFA2
• SFA-2activating transcription factor B
• SF-HT-activated gene 2 protein

Background

BATF is encoded by this gene is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BATF