BAP1 Antibody (1G8) [Biotin]

• Reactivity: Hu
• Applications: WB, Flow
• Clone: 1G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

BAP1 Antibody (1G8) [Biotin] Summary

• Immunogen: Full-length human BAP1 protein expressed and purified from E.coli. [UniProt# Q92560]
• Epitope: Within the UCH domain of BAP1.
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for BAP1 Antibody (1G8) [Biotin]

• BAP1
• BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
• BRCA1-associated protein 1
• Cerebral protein 6
• cerebral protein-13
• Cerebral Protein-6
• DKFZp686N04275
• EC 3.4.19.12
• FLJ35406
• FLJ37180
• hucep-6
• KIAA0272HUCEP-13
• ubiquitin carboxyl-terminal hydrolase BAP1
• ubiquitin carboxy-terminal hydrolase
• UCHL2

Background

Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAP1