BAP1 Antibody (1G8)

Images

 
Western Blot: BAP1 Antibody (1G8) [NB110-60521] - Detection of BAP1 in HeLa WCE.
Flow Cytometry: BAP1 Antibody (1G8) [NB110-60521] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using BAP1 antibody (dark blue). Isotype control shown in orange. An antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
1G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

BAP1 Antibody (1G8) Summary

Immunogen
Full-length human BAP1 protein expressed and purified from E.coli. [UniProt# Q92560]
Epitope
Within the UCH domain of BAP1.
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BAP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 1 ug per million cells
Application Notes
This BAP1 antibody is useful for Western blot. In Western blot a band is seen at ~85 kDa, representing the BAP1 protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
BAP1 Lysate (NBL1-07915)
Publications
Read Publication using
NB110-60521 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BAP1 Antibody (1G8)

  • BAP1
  • BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
  • BRCA1-associated protein 1
  • Cerebral protein 6
  • cerebral protein-13
  • Cerebral Protein-6
  • DKFZp686N04275
  • EC 3.4.19.12
  • FLJ35406
  • FLJ37180
  • hucep-6
  • KIAA0272HUCEP-13
  • ubiquitin carboxyl-terminal hydrolase BAP1
  • ubiquitin carboxy-terminal hydrolase
  • UCHL2

Background

BAP1 (BRCA1-associated protein 1) was initially identified as BRCA1 binding protein and subsequently it was found to be a nuclear deubiquitinating enzyme belonging to ubiquitin C-terminal hydrolase family that play a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. BAP1 forms the catalytic component of PR-DUB complex (BAP1 and ASXL1) that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). BAP1 regulates cell growth by mediating deubiquitination of HCFC1 N- and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. BAP1 interferes with activity of BRCA1-BARD1 heterodimer by inhibiting their ability to mediate ubiquitination/autoubiquitination. BAP1 is considered a tumour suppressor and defects in BAP1 gene have been linked to tumor predisposition syndrome (TPDS). Germline mutations of BAP1 confers increased susceptibility for certain malignancies including uveal melanoma, epithelioid atypical Spitz tumours, cutaneous melanoma, and mesothelioma. Somatic BAP1 mutations are linked to cutaneous melanocytic tumours, uveal melanoma, mesothelioma, clear cell renal cell carcinoma etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow

Publications for BAP1 Antibody (NB110-60521)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

View specific protocols for BAP1 Antibody (NB110-60521): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BAP1 Antibody (NB110-60521) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-60521AF405
Alexa Fluor 488 Labeled NB110-60521AF488
Alexa Fluor 647 Labeled NB110-60521AF647
Alexa Fluor 700 Labeled NB110-60521AF700
Allophycocyanin Labeled NB110-60521APC
Biotin Labeled NB110-60521B
DyLight 350 Labeled NB110-60521UV
DyLight 405 Labeled NB110-60521V
DyLight 488 Labeled NB110-60521G
DyLight 550 Labeled NB110-60521R
DyLight 650 Labeled NB110-60521C
DyLight 680 Labeled NB110-60521FR
DyLight 755 Labeled NB110-60521IR
FITC Labeled NB110-60521F
HRP Labeled NB110-60521H
PE Labeled NB110-60521PE

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Bioinformatics

Gene Symbol BAP1
Entrez
Uniprot