BAP1 Antibody (1G8) [FITC]

• Reactivity: Hu
• Applications: WB, Flow
• Clone: 1G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

BAP1 Antibody (1G8) [FITC] Summary

• Immunogen: Full-length human BAP1 protein expressed and purified from E.coli. [UniProt# Q92560]
• Epitope: Within the UCH domain of BAP1.
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for BAP1 Antibody (1G8) [FITC]

• BAP1
• BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
• BRCA1-associated protein 1
• Cerebral protein 6
• cerebral protein-13
• Cerebral Protein-6
• DKFZp686N04275
• EC 3.4.19.12
• FLJ35406
• FLJ37180
• hucep-6
• KIAA0272HUCEP-13
• ubiquitin carboxyl-terminal hydrolase BAP1
• ubiquitin carboxy-terminal hydrolase
• UCHL2

Background

BAP1 (BRCA1-associated protein 1) was initially identified as BRCA1 binding protein and subsequently it was found to be a nuclear deubiquitinating enzyme belonging to ubiquitin C-terminal hydrolase family that play a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. BAP1 forms the catalytic component of PR-DUB complex (BAP1 and ASXL1) that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). BAP1 regulates cell growth by mediating deubiquitination of HCFC1 N- and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. BAP1 interferes with activity of BRCA1-BARD1 heterodimer by inhibiting their ability to mediate ubiquitination/autoubiquitination. BAP1 is considered a tumour suppressor and defects in BAP1 gene have been linked to tumor predisposition syndrome (TPDS). Germline mutations of BAP1 confers increased susceptibility for certain malignancies including uveal melanoma, epithelioid atypical Spitz tumours, cutaneous melanoma, and mesothelioma. Somatic BAP1 mutations are linked to cutaneous melanocytic tumours, uveal melanoma, mesothelioma, clear cell renal cell carcinoma etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BAP1