BAMBI/NMA Antibody

Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2387) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAMBI/NMA at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockdown of BAMBI inhibited myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The western blot images of BAMBI and GAPDH; (b) the efficiency of siRNA interference on the mRNA and protein expression of BAMBI; (c) the mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (d) the western blot images of MyoD at 48 h, MyHC at 96 h, and their corresponding GAPDH; (e) the protein expression of MyoD at 48 h and MyHC at 96 h; (f) immunofluorescence of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (g) the populations of myotubes; (h) the differentiation index; and (i) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.

Knockdown of BAMBI suppressed Wnt/ beta -catenin signaling. All the cell samples were harvested after transfection and myogenic induction for 48 h. (a) The western blot images of nuclear beta -catenin and laminB1; (b) the nuclear beta -catenin protein levels; (c) the mRNA expression of Axin2; (d) the western blot images of nuclear beta -catenin and laminB1; (e) the nuclear beta -catenin protein levels; (f) the mRNA expression of Axin2; (g) the western blot images of BAMBI and GAPDH; and (h) the mRNA and protein expression of BAMBI. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.

Rapamycin-induced autophagy in HUVEC.Western blot for BAMBI, GAPDH, and LC3 in BAMBI overexpressing HUVECs cultured in 10% FBS and treated with vehicle (CTRL), rapamycin, bafilomycin or bafilomycin plus rapamycin for 24 hours. Rapamycin alone reduces the BAMBI band, whereas bafilomycin alone and even in combination with rapamycin enhances it. Rapamycin causes a shift from the LC3-I to the LC3-II band, and bafilomycin alone enhances both LC3 bands. Bafilomycin does not, however, prevent the shift from LC3-I to LC3-II induced by rapamycin. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20886049), licensed under a CC-BY license. Not internally tested by R&D Systems.

BAMBI protein levels in overexpressing HUVECs.7A. Representative Western blot and densitometry analysis for BAMBI in HUVECs overexpressing BAMBI (lentiviral system). Cells were cultured in 10% FBS or 0.2% FBS for 24 hours prior to protein extraction. GAPDH is shown as loading control. 7B. For densitometry analysis, normalized relative intensities were calculated from band intensity values obtained using the NIH Image J program. Data are means +/− SEM of relative BAMBI∶GAPDH ratios (normalized to 10% FBS control cells) for two independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20886049), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BAMBI/NMA Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse BAMBI/NMA
  Ile28-Thr143
  Accession # Q9D0L6
• Specificity: Detects mouse BAMBI/NMA in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human BAMBI is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BAMBI
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAMBI/NMA Antibody

• BAMBI
• BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)
• BMP and activin membrane-bound inhibitor homolog
• NMA
• NMANon-metastatic gene A protein
• Putative transmembrane protein NMA

Background

BAMBI/NMA is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family protein but has a short cytoplasmic region that lacks the kinase domain. It functions as a membrane-bound decoy receptor by competing with type I receptors to form a heterodimer with type II receptors. Mouse BAMBI/NMA share approximately 93% amino acid sequence homology with human BAMBI/NMA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAMBI/NMA Antibody (AF2387)(6)

Publications using AF2387

• Yuan CL, Liang R, Liu ZH et al. Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-B/epithelial-mesenchymal transition signaling pathway Exp Ther Med 6/1/2018 [PMID: 29805551]
• Xiangping Yao, Taiyong Yu, Chen Zhao, Youlei Li, Ying Peng, Fengxue Xi et al. Evodiamine promotes differentiation and inhibits proliferation of C2C12 muscle cells International Journal of Molecular Medicine 12/13/2017 [PMID: 29286060]
• Qiangling Zhang, Xin-E Shi, Chengchuang Song, Shiduo Sun, Gongshe Yang, Xiao Li BAMBI Promotes C2C12 Myogenic Differentiation by Enhancing Wnt/ beta -Catenin Signaling International Journal of Molecular Sciences 8/3/2015 [PMID: 26247931]
• Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. PLoS ONE, 2010-09-24;5(9):e12995. 2010-09-24 [PMID: 20886049] (IHC-Fr, Western Blot, Mouse)
• Tramullas M, Lantero A, Diaz A, Morchon N, Merino D, Villar A, Buscher D, Merino R, Hurle JM, Izpisua-Belmonte JC, Hurle MA BAMBI (bone morphogenetic protein and activin membrane-bound inhibitor) reveals the involvement of the transforming growth factor-beta family in pain modulation. J. Neurosci., 2010-01-27;30(4):1502-11. 2010-01-27 [PMID: 20107078] (IHC-P, Mouse)
• Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF TLR4 enhances TGF-beta signaling and hepatic fibrosis. Nat. Med., 2007-10-21;13(11):1324-32. 2007-10-21 [PMID: 17952090] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: BAMBI
• Uniprot:
  • Q9D0L6()