BAK Antibody

Images

 
Western Blot: BAK Antibody [NB100-56092] - Analysis of Bak in Daoy whole cell lysate using anti-Bak antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Bak Antibody [NB100-56092] - Formalin-fixed paraffin-embedded tissue sections of human heart with cardiomyopathy stained for Bak expression using this antibody at 1:2000 (A and B). ...read more
Immunohistochemistry-Paraffin: Bak Antibody [NB100-56092] - Formalin-fixed paraffin-embedded human tissue for Bak expression using this antibody at 1:2000. A. Ductal breast carcinoma. B. Lymphocyte infiltration in soft ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BAK Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 16-36 (EPALPSASEEQVAQDTEEVFR) of human Bak was used as immunogen, GenBank no. NP_001179.1.
Specificity
BAK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAK1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
Control
Spleen Lysate (NB820-59259)
BAK Lysate (NBL1-07909)
Lymph Node Lysate (NB820-59242)
BAK Lysate (NBP2-65116)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56092 in the following applications:

Publications
Read Publication using
NB100-56092 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for BAK Antibody

  • Apoptosis regulator BAK
  • BAK
  • BAK1
  • BAK-LIKE
  • BAKMGC3887
  • BCL2-antagonist/killer 1
  • BCL2L7
  • bcl2-L-7
  • BCL2L7bcl-2 homologous antagonist/killer
  • BCL2-like 7 protein
  • Bcl-2-like protein 7
  • CDN1
  • MGC117255
  • pro-apoptotic protein BAK

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. NB100-56092 recognizes BAK. Human BAK is a 211 amino acid protein, GenBank no. NP_001179.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAK Antibody (NB100-56092)(1)

We have publications tested in 3 confirmed species: Mouse, Rat, Canine.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(1)
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Rat
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Review for BAK Antibody (NB100-56092) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56092:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot BAK NB100-56092
Enlarge
reviewed by:
Anonymous
WB Human 07/14/2016
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from Daoy cells
SpeciesHuman

Blocking

Blocking Details5% Milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:2000 in 5% milk PBST overnight at 4 degrees C

Secondary Antibody

Secondary DescriptionDonkey anti Rabbit IgG HRP
Secondary Manufacturer Cat#Southern Biotech 6440-05
Secondary Concentration1:5000

Details

Detection NotesAdvansta WesternBright ECL

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for BAK Antibody (NB100-56092) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for BAK Antibody (NB100-56092)

Discover related pathways, diseases and genes to BAK Antibody (NB100-56092). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for BAK Antibody (NB100-56092)

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Pathways for BAK Antibody (NB100-56092)

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PTMs for BAK Antibody (NB100-56092)

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Research Areas for BAK Antibody (NB100-56092)

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Blogs on BAK.

IRE1 alpha dependent apoptotic-signaling pathway
Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Dynamin-related Protein 1 (DRP1) in Mitochondria and Apoptosis.
Dynamin-related Protein 1 (DRP1) is known to function in mitochondrial and peroxisomal division and mediate membrane fission through oligomerization into ring-like structure and sever the mitochondrial membrane, through a GTP hydrolysis-dependent mech...  Read full blog post.

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Recent Reviews

4
5
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4
1
3
0
2
0
1
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Anonymous
07/14/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol BAK1
Entrez