ELMO1 Antibody

Western Blot: ELMO1 Antibody [NBP1-33645] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ELMO1 Antibody [NBP1-33645] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: ELMO1 Antibody [NBP1-33645] - Paraffin-embedded Stomach, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ELMO1 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ELMO1. The exact sequence is proprietary.
• Localization: Cytoplasm, Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELMO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0) and 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for ELMO1 Antibody

• ced-12 homolog 1
• CED12CED-12
• ELMO-1
• engulfment and cell motility 1 (ced-12 homolog, C. elegans)
• engulfment and cell motility 1
• engulfment and cell motility protein 1
• KIAA0281MGC126406
• Protein ced-12 homolog

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ELMO1