ELMO1 Antibody - BSA Free

Western Blot: ELMO1 Antibody [NBP1-33645] - ELMO1 antibody detects ELMO1 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 7.5% SDS-PAGE. ELMO1 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ELMO1 Antibody [NBP1-33645] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: ELMO1 Antibody [NBP1-33645] - Human stomach, using ELMO1antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: ELMO1 Antibody [NBP1-33645] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.

Western Blot: ELMO1 Antibody [NBP1-33645] - ELMO1 antibody detects ELMO1 protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 7.5% SDS-PAGE. ELMO1 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ELMO1 Antibody [NBP1-33645] - Sample (30 ug of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Ze, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ELMO1 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ELMO1. The exact sequence is proprietary.
• Localization: Cytoplasm, Cell membrane
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELMO1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ELMO1 Antibody - BSA Free

• ced-12 homolog 1
• CED12CED-12
• ELMO-1
• engulfment and cell motility 1 (ced-12 homolog, C. elegans)
• engulfment and cell motility 1
• engulfment and cell motility protein 1
• KIAA0281MGC126406
• Protein ced-12 homolog

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ELMO1