CD97 Antibody - BSA Free

Western Blot: CD97 Antibody [NBP2-24552] - Analysis of human Leukocyte Antigen CD97 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) NIH 3T3 cell lysate using NBP2-24552 at 4 ug/ml.

Immunohistochemistry-Paraffin: CD97 Antibody [NBP2-24552] - IHC analysis of human spleen using NBP2-24552 at 10 ug/ml.

Simple Western: CD97 Antibody [NBP2-24552] - Simple Western lane view shows a specific band for CD97 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Pm, Rt, Pm, Ma-Op, RM
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

CD97 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CD97 Antibody - BSA Free (NBP2-24552) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-CD97 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 220-270 of human Leukocyte Antigen CD97 was used as the immunogen
• Predicted Species: Rat (100%), Chimpanzee (100%), Rhesus Macaque (100%), Opossum (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADGRE5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Simple Western 1:10
  • Western Blot 3-5 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 81 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CD97 Antibody - BSA Free

• CD97 antigenseven-span transmembrane protein
• CD97 molecule
• Leukocyte antigen CD97
• seven transmembrane helix receptor
• seven-transmembrane, heterodimeric receptor associated with inflammation
• TM7LN1

Background

CD97, a CD55 Receptor, is a leukocyte antigen located on most activated leukocytes. CD97 is highly homologous to EMR2 with five EGF-like domains in the N-terminal extracellular domain, suggesting that it may be involved in cell signaling as well as in cell adhesion (Hamann et al., 1996). Two variants are produced by alternative splicing, where the shorter form lacks exons 5 and 6 and reduces the number of EGF-like domains from 5 to 3, compared to the full-length isoform. CD97 is associated with with the activated lesions of multiple sclerosis. CD97 expression is also implicated in the dedifferentiation of colon and thyroid tumors. CD97 is expressed abundantly in cells of hematopoietic origin and is markedly upregulated on activated T and B cells. ESTs have been isolated from a wide variety of tissue libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD97 Antibody (NBP2-24552)(2)

Publications using NBP2-24552

• He Y, Xu L, Feng M, Wang W. Role of CD97 small isoform in human cervical carcinoma Int J Exp Pathol 2019-03-18 [PMID: 30883974] (WB, Human)
• Becker S, Wandel E, Wobus M et al. Overexpression of CD97 in intestinal epithelial cells of transgenic mice attenuates colitis by strengthening adherens junctions. PLoS One. 2010-01-13 [PMID: 20084281]

Bioinformatics

• Gene Symbol: ADGRE5