Bag-1 Antibody (GP3.10G3E2) [Unconjugated]

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Bag-1 at approximately 36 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Bag-1 was immunoprecipitated from lysates (5 x 106cells) of THP-1 human acute monocytic leukemia and HEK293 human embryonic kidney cell line following incubation with 3 µg Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) for 1-16 hours at 4 °C. Bag-1-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bag-1 (left panel) was detected by Western blot using 0.5 µg/mL Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852). View our recommended buffer recipes for immunoprecipitation.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bag-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Bag-1 at approximately 29, 39, and 50 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP, KO
• Clone: GP3.10G3E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Bag-1 Antibody (GP3.10G3E2) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Bag-1
• Specificity: Detects the 36 kDa and 50 kDa isoforms of human Bag-1. Does not detect mouse Bag-1.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAG1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 0.6 ug/10^6 cells
  • Knockout Validated
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bag-1 Antibody (GP3.10G3E2) [Unconjugated]

• BAG family molecular chaperone regulator 1
• Bag1
• Bag-1
• Bcl-2 associating athanogene-1 protein
• Bcl-2-associated athanogene 1
• BCL2-associated athanogene
• Bcl-2-binding protein
• glucocortoid receptor-associated protein RAP46,46-KD
• HAP
• RAP46

Background

Bag-1 (Bcl-2-associated Athanogene 1) is a 31-kDa protein with an apparent molecular mass of 46 kDa that can bind to Bcl-2 and enhance its anti-apoptotic effect, resulting in increased protection from cell death induced by apoptotic stimuli. Bag-1 shares no significant homology with Bcl-2 or other Bcl-2 family proteins. The C-terminal region of Bag-1 binds the PDGF and HGF Receptor, and enhances growth factor-mediated protection from apoptosis. This represents a link between growth factor receptors and anti-apoptotic mechanisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bag-1 Antibody (MAB852)(1)

Publication using MAB852

• Pan MH, Huang YT, Ho CT, Chang CI, Hsu PC, Sun Pan B Induction of apoptosis by Meretrix lusoria through reactive oxygen species production, glutathione depletion, and caspase activation in human leukemia cells. Life Sci., 2006-04-26;79(12):1140-52. 2006-04-26 [PMID: 16730358] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BAG1