BAF57 Antibody

Images

 
Western Blot: BAF57 Antibody [NBP1-31100] - Raji 7.5% SDS PAGE; antibody diluted at 1:3000
Immunocytochemistry/ Immunofluorescence: BAF57 Antibody [NBP1-31100] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BAF57 protein stained by BAF57 antibody C-term diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-31100] - Paraffin-embedded lung, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.04 mg/ml

Order Details

BAF57 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BAF57. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1.04 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAF57 Antibody

  • BAF57BRG1-associated factor 57
  • chromatin remodeling complex BRG1-associated factor 57
  • subfamily e, member 1
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMARCE1