BAF57 Antibody - BSA Free

Western Blot: BAF57 Antibody [NBP1-31100] - Raji 7.5% SDS PAGE; antibody diluted at 1:3000

Immunocytochemistry/Immunofluorescence: BAF57 Antibody [NBP1-31100] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BAF57 protein stained by BAF57 antibody C-term diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: BAF57 Antibody [NBP1-31100] - Paraffin-embedded lung, using antibody at 1:100 dilution.

Western Blot: BAF57 Antibody [NBP1-31100] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BAF57 antibody [C3], C-term (NBP1-31100) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BAF57 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BAF57 Antibody - BSA Free (NBP1-31100) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BAF57. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCE1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BAF57 Antibody - BSA Free

• BAF57BRG1-associated factor 57
• chromatin remodeling complex BRG1-associated factor 57
• subfamily e, member 1
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SMARCE1