BAF57 Antibody - BSA Free

Western Blot: BAF57 Antibody [NB100-2591] - Detection of Human and Mouse BAF57/SMARCE1 on HeLa whole cell lysates using NB100-2591.

Immunohistochemistry: BAF57 Antibody [NB100-2591] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- BAF57/SMARCE1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

BAF57 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 360 and the C-terminus (residue 411) of human BAF57/SMARCE1using the numbering given in entry NP_003070.3 (GeneID 6605).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 to 1:2000
  • Immunohistochemistry-Paraffin 1:500 to 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: This antibody is useful for Western Blot and immunoprecipitation. Prepare working dilution immediately before use.
• Control:

  BAF57 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BAF57 Antibody - BSA Free

• BAF57BRG1-associated factor 57
• chromatin remodeling complex BRG1-associated factor 57
• subfamily e, member 1
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAF57 Antibody (NB100-2591)(4)

Publications using NB100-2591

• Giessrigl B, Schmidt WM, Kalipciyan M et al. Fulvestrant induces resistance by modulating GPER and CDK6 expression: implication of methyltransferases, deacetylases and the hSWI/SNF chromatin remodelling complex. Br J Cancer. 2013-11-12 [PMID: 24169358] (WB, Human)
• erson DJ, Vargas JD, Hsiao JP et al. Recruitment of functionally distinct membrane proteins to chromatin mediates nuclear envelope formation in vivo. J Cell Biol;186(2):183-191. 2009-01-01 [PMID: 19620630]
• Luo B, Cheung HW, Subramanian A et al. Highly parallel identification of essential genes in cancer cells. Proc Natl Acad Sci U S A 2008-12-17 [PMID: 19091943]
• Link KA, Balasubramaniam S, Sharma A et al. Targeting the BAF57 SWI/SNF subunit in prostate cancer: a novel platform to control androgen receptor activity. Cancer Res 2008-06-01 [PMID: 18559499]

Bioinformatics

• Gene Symbol: SMARCE1
• Entrez:
  • 6605(Human)
  • 57376(Mouse)
• Uniprot:
  • Q969G3(Human)