BAF57 Antibody

Western Blot: BAF57 Antibody [NB100-1297] - Western blot analysis of BAF57 using NB100-1297 in Jurkat lysate (RIPA buffer, 35ug total protein per lane) at 0.2ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: BAF57 Antibody [NB100-1297] - In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells.

Western Blot: BAF57 Antibody [NB100-1297] - A) HEK293 overexpressing BAF57 and probed with (mock transfection in first lane). B) see Western Blot.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BAF57 Antibody Summary

• Immunogen: Peptide with sequence C-PPTDPIPEDEKKE corresponding to C-Terminus according to NP_003070.3.
• Localization: Nuclear
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SMARCE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.2 - 0.6 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in lysates of HEPG2, HeLa and Jurkat (calculated MW of 46.6 kDa band according to NP_003070.3). A 48 kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. IHC-P: Human prostate shows nuclear staining in secretory epithelial cells.
• Control:

  Jurkat Whole Cell Lysate
  HeLa Whole Cell Lysate
  BAF57 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BAF57 Antibody

• BAF57BRG1-associated factor 57
• chromatin remodeling complex BRG1-associated factor 57
• subfamily e, member 1
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily E member 1

Background

The SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. The complex contains an ATP-dependent nucleosome disruption activity that can lead to enhanced binding of transcription factors. The BRG1/brm associated factors, or BAF, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF. A 57kD BAF subunit, BAF57, is present in higher eukaryotes, but not in yeast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAF57 Antibody (NB100-1297)(1)

Publication using NB100-1297

• Wang W, Chi T, Xue Y et al. Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes. Proc Natl Acad Sci U S A 1998-01-20 [PMID: 9435219]

Bioinformatics

• Gene Symbol: SMARCE1
• Entrez:
  • 6605(Human)
  • 57376(Mouse)
• Uniprot:
  • NP_003070.(Human)