BAF180/PB1 Antibody (BL-39-2C3)

Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Whole cell lysate (50 ug) from Ba/F3 and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 10 seconds.

Simple Western: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of mouse PBRM1 by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (NBP2-76400) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally..

Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.

Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti- PBRM1 recombinant monoclonal antibody [BL-39-2C3] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of human PBRM1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (NBP2-76400).

Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of human PBRM1 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal [BL-3-2F2] (NBP2-76400) and rabbit anti-PBRM1 (NB100-1559 and NB100-1761).

Flow Cytometry: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinantmonoclonal [BL-39-2C3] or isotypecontrol (unshaded). Secondary: DyLight® 488-conjugatedgoat anti-rabbit IgG.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, mIF
• Clone: BL-39-2C3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

BAF180/PB1 Antibody (BL-39-2C3) Summary

• Description: Novus Biologicals Rabbit BAF180/PB1 Antibody (BL-39-2C3) (NBP2-76400) is a recombinant monoclonal antibody validated for use in Multiplex Immunofluorescence, IHC, WB, Flow, ICC/IF, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein representing residues 1-293 of human Polybromo 1 (NP_060783.3 )
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PBRM1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Fixed and permeabilized cells were stained with 0.8 µl per 1 x 10^6
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 5 µl/mg lysate
  • Multiplex Immunofluorescence
  • Simple Western 1:50-1:250
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  See Simple Western Antibody Database for Simple Western validation: Tested in 3T3 [0.08 mg/mL], separated by Size, antibody dilution of 1:250, 1:50, apparent MW was 195-196 kDa

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for BAF180/PB1 Antibody (BL-39-2C3)

• BAF180
• BAF180/PB1
• BAF180PB1BRG1-associated factor 180
• hPB1
• MGC156155
• MGC156156
• PB1
• PBRM1
• polybromo 1
• Polybromo-1D
• protein polybromo-1

Background

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PBRM1 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PBRM1 has been proposed to function as a tumor suppressor protein. Recently, PBRM1 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBRM1