Bad Antibody - (cleaved)

Western Blot: cleaved BAD Antibody [NB100-56602] - Western blot analysis of Bad-cv using NB100-56602 at 1:500 against lysate of (A) untreated HeLa cells and (B) HeLa cells treated with TNFa for 2 hrs.

Product Discontinued

Bad Antibody - (cleaved) Summary

• Immunogen: This antibody was developed by immunizing rabbits with a synthetic peptide containing Asp(cleavage site) of Bad.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Western Blot 1:250 - 1:500
• Application Notes: Use in Flow Intracellular reported in scientific literature (PMID 24804954)
• Control:

  Bad Overexpression Lysate

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 0.1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Bad Antibody - (cleaved)

• Bad
• BBC2
• BBC6
• bcl2 antagonist of cell death
• BCL2-antagonist of cell death protein
• BCL2-associated agonist of cell death
• Bcl-2-binding component 6
• BCL2-binding component 6
• BCL2-binding protein
• BCL2L8
• Bcl2-L-8
• BCL2L8bcl2-L-8
• Bcl-2-like protein 8
• BCL-X/BCL-2 binding protein
• Bcl-XL/Bcl-2-associated death promoter

Background

The function of Bad, a proapoptotic member of the Bcl-2 family, is regulated primarily by rapid changes in phosphorylation that modulate its protein-protein interactions and subcellular localization. Human Bad, which is 43 amino acids shorter than its mouse counterpart, is cleaved by a caspase upon exposure of Jurkat T cells to anti-FAS antibody, tumor necrosis factor alpha (TNF-alpha), or TRAIL. Moreover, a truncated form of human Bad lacking the N-terminal 28 amino acids is more potent than wild-type Bad in inducing apoptosis. Bad plays a novel and important role in maintaining the apoptotic phenotype in response to various apoptosis inducers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bad Antibody (NB100-56602)(1)

Publication using NB100-56602

• Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014-05-07 [PMID: 24804954] (Flow Cytometry Control, Human)
  
  Details:
  Mouse splenocytes, human PBMC, Jurkat, EL4: Fig 5. GADPH was detected at ~36 kDa.

Bioinformatics

• Gene Symbol: BAD
• Entrez:
  • 572(Human)
• Uniprot:
  • Q92934(Human)