B7-H4 Antibody [Biotin]

HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H4 or (B) irrelevant protein and eGFP was stained with Goat Anti-Human/Mouse B7-H4 Biotinylated Polyclonal Antibody (Catalog # BAF2154) followed by APC-conjugated Streptavidin (F0050). Quadrant markers were set based on Goat IgG control antibody staining (Catalog # BAF108). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

B7-H4 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse B7-H4
  Phe29-Pro258
  Accession # Q7TSP5
• Specificity: Detects mouse B7-H4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H2, rmB7-H3, and rmPD-L2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VTCN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H4 Antibody [Biotin]

• B7h.5
• B7H4
• B7-H4
• B7H4T-cell costimulatory molecule B7x
• B7S1
• B7S1VCTN1
• B7x
• B7XPRO1291
• FLJ22418
• Immune costimulatory protein B7-H4
• Protein B7S1
• T cell costimulatory molecule B7x
• V-set domain containing T cell activation inhibitor 1
• V-set domain-containing T-cell activation inhibitor 1
• Vtcn1

Background

B7-H4, also known as B7x and B7S1, is a member of the B7 family of immune co-stimulatory proteins (1). Mature B7-H4 is a 50 kDa - 80 kDa glycosylated molecule with a 28 kDa protein core (2). Partial sensitivity to cleavage by PI-PLC suggests a possible GPI linkage of mouse B7-H4 to the cell membrane (3). The 230 amino acid (aa) extracellular region of B7-H4 contains one Ig-like V-set domain and one Ig-like C2-set domain which is followed by a hydrophobic C-terminal region (3 - 5). Within the ECD, mouse B7-H4 shares 90% and 99% aa sequence identity with human and rat B7-H4, respectively. It shares 21% - 29% aa sequence identity with B7-1, B7-2, B7-H1, B7-H2, B7-H3, and PD-L2. B7-H4 expression is induced on mitogen- or LPS-activated B cells, T cells, dendritic cells, monocytes, and macrophages (3, 4) and blocked by GM-CSF or IL-4 (6). It is also expressed on various normal epithelia and upregulated in several carcinomas and renal tubule epithelial cell lesions (2, 6 - 10). B7-H4 is expressed on the surface of macrophages but intracellularly in ovarian and breast cancer cells (2, 6 - 7). It is found in the serum and ascites fluid of cancer patients (11). B7-H4 binds an unidentified ligand on activated T cells which is distinct from BTLA, CD28, CTLA4, ICOS, and PD-1 (3 - 5, 12). Exposure to B7-H4 inhibits antigen-dependent induction of T cell proliferation and activation (3 - 5). Alternatively, B7-H4 expressing renal tubular epithelial cells promote T cell activation (8). Regulatory T cells, IL-6, and IL-10 induce B7-H4 expression on antigen presenting cells which fosters tumor growth by dampening the anti-tumor immune response (6, 13). B7-H4 also promotes the malignant transformation of epithelial cells by protecting them from apoptosis (2). Despite evidence for the involvement of B7-H4 in immune regulation, mice deficient in its expression do not show significant immune deficiencies, suggesting compensation by other molecules in vivo (14).

1. Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515.
2. Salceda, S. et al. (2005) Exp. Cell Res. 306:128.
3. Prasad, V.R. et al. (2003) Immunity 18:863.
4. Sica, G.L. et al. (2003) Immunity 18:849.
5. Zang, X. et al. (2003) Proc. Natl. Acad. Sci. 100:10388.
6. Kryczek, I. et al. (2006) J. Exp. Med. 203:871.
7. Tringler, B. et al. (2005) Clin. Cancer Res. 11:1842.
8. Chen, Y. et al. (2006) Kidney Int. 70:2092.
9. Choi, I.-H. et al. (2003) J. Immunol. 171:4650.
10. Krambeck, A.E. et al. (2006) Proc. Natl. Acad. Sci. 103:10391.
11. Simon, I. et al. (2006) Cancer Res. 66:1570.
12. Sedy, J.R. et al. (2005) Nat. Immunol. 6:90.
13. Kryczek, I. et al. (2006) J. Immunol. 177:40.
14. Suh, W.-K. et al. (2006) Mol. Cell. Biol. 26:6403.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-H4 Antibody (BAF2154)(2)

Publications using BAF2154

• Lee J, Scandiuzzi L, Ray A, Wei J, Hofmeyer K, Abadi Y, Loke P, Lin J, Yuan J, Serreze D, Allison J, Zang X B7x in the periphery abrogates pancreas-specific damage mediated by self-reactive CD8 T cells. J Immunol, 2012-09-12;189(8):4165-74. 2012-09-12 [PMID: 22972920] (Flow Cytometry, IHC-P, Mouse)
• Hofmeyer , Kimberly, Scandiuzzi , Lisa, Ghosh , Kaya, Pirofski , Liise-An, Zang , Xingxing Tissue-expressed B7x affects the immune response to and outcome of lethal pulmonary infection. J Immunol, 2012-08-01;189(6):3054-63. 2012-08-01 [PMID: 22855708] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: VTCN1
• Uniprot:
  • Q7TSP5()