B7-H4 Antibodies

HAF017). Specific bands were detected for B7-H4 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for B7-H4 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse placenta tissue, mouse uterus tissue, and SK-BR-3 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse B7-H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for B7-H4 at approximately 50-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0108). Quadrant markers were set based on Goat IgG control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0108). Quadrant markers were set based on Goat IgG control antibody staining (Catalog # AB-108-C). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

HAF008). Specific bands were detected for B7-H4 at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). Specific bands were detected for B7-H4 at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of SK-BR-3 human breast cancer cell line, mouse uterus tissue, and rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat B7-H4 Monoclonal Antibody (Catalog # MAB21542) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for B7-H4 at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF008). A specific band was detected for B7-H4 at approximately 50-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for B7-H4 at approximately 50-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse uterus tissue, mouse placenta tissue, and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse B7-H4 Monoclonal Antibody (Catalog # MAB21541) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for B7-H4 at approximately 50-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF008). A specific band was detected for B7-H4 at approximately 50-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for B7-H4 at approximately 50-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of SK-BR-3 human breast cancer cell line and T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human B7-H4 Monoclonal Antibody (Catalog # MAB65764) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for B7-H4 at approximately 50-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0113). Quadrant markers were set based on Rat IgG2b control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). Quadrant markers were set based on Rat IgG2b control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) mouse B7-H4 or (B) irrelevant protein and eGFP was stained with Rat Anti-Human/Mouse B7-H4 Monoclonal Antibody (Catalog # MAB2154) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on Rat IgG2b control antibody staining (Catalog # MAB0061). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human B7-H4 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human B7-H4 Monoclonal Antibody (Catalog # MAB65761) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0101B).View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B).View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) human B7-H4 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human B7-H4 Monoclonal Antibody (Catalog # MAB65761R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0101B)." class="big_lightbox"> F0101B)." alt="HEK293 human embryonic kidney cell line transfected with either (A) human B7-H4 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human B7-H4 Monoclonal Antibody (Catalog # MAB6576) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />