B7-H3/CD276 Antibody (6A1)

Western Blot: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Western blot analysis using CD276 mAb against HEK293 (1) and CD276(AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Analysis of cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 mouse mAb with DAB staining.

Flow Cytometry: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Analysis of PC-3 cells using CD276 mouse mAb (green) and negative control (purple).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 6A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

B7-H3/CD276 Antibody (6A1) Summary

• Immunogen: Purified recombinant fragment of human B7-H3/CD276 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD276
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for B7-H3/CD276 Antibody (6A1)

• B7H3
• B7-H3
• B7H34Ig-B7-H3
• B7-H3B7 homolog 3
• CD276 antigen
• CD276 molecule
• CD276
• Costimulatory molecule

Background

Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD276