Home » B7-H2/ICOSLG » B7-H2/ICOSLG Antibodies » B7-H2/ICOSLG Antibody (599841) [Unconjugated]

B7-H2/ICOSLG Antibody (599841) [Unconjugated]

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A20 mouse B cell lymphoma cell line was stained with Mouse B7‑H2 Monoclonal Antibody (Catalog # MAB158, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).
A20 mouse B cell lymphoma cell line was stained with Mouse B7‑H2 Monoclonal Antibody (Catalog # MAB158, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by ...read more
B7‑H2 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B7‑H2 Monoclonal Antibody (Catalog # MAB158) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
599841
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB158U-100UG
Alexa Fluor 405
FAB158V-100UG
Alexa Fluor 488
FAB158G-100UG
Alexa Fluor 594
FAB158T-100UG
Alexa Fluor 647
FAB158R-100UG
Alexa Fluor 700
FAB158N-100UG
Alexa Fluor 750
FAB158S-100UG
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB158-SP
Carrier Free
FAB158V-100UG
Carrier Free
FAB158U-100UG
Carrier Free
FAB158T-100UG
Carrier Free
FAB158S-100UG
Carrier Free
FAB158R-100UG
Carrier Free
FAB158N-100UG
Carrier Free
FAB158G-100UG
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B7-H2/ICOSLG Antibody (599841) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H2        
Glu47-Lys279
Accession # Q9JHJ8
Specificity
Detects mouse B7-H2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-H2, rhB7-H3,
rhB7-H3b, rhPD-L2, recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H3, rmB7-H4, rmPD-L2, or recombinant rat B7-2 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
Icosl
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H2/ICOSLG Antibody (599841) [Unconjugated]

  • B7H2
  • B7-H2
  • B7H2B7 homolog 2
  • B7-H2B7-related protein 1
  • B7RP-1
  • B7RP1B7-like protein Gl50
  • B7RP-1LICOS
  • CD275 antigen
  • CD275
  • GL50
  • GL50transmembrane protein B7-H2 ICOS ligand
  • ICOSL
  • ICOS-L
  • ICOSLG
  • inducible T-cell co-stimulator ligand
  • KIAA0653ICOS ligand

Background

B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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B7-H2/ICOSLG
Antibody (599841) [Un ...
MAB158
B7-H2/ICOSLG Antibody (599841) [Unconjugated]
Species: Mu
Applications: Flow, CyTOF-ready, ICC/IF

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB158U
Alexa Fluor 405 FAB158V
Alexa Fluor 488 FAB158G
Alexa Fluor 594 FAB158T
Alexa Fluor 647 FAB158R
Alexa Fluor 700 FAB158N
Alexa Fluor 750 FAB158S
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol Icosl
Uniprot
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