B7-H2/ICOSLG Antibody (599841) [Unconjugated]

A20 mouse B cell lymphoma cell line was stained with Mouse B7‑H2 Monoclonal Antibody (Catalog # MAB158, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).

B7-H2 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB158) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 599841
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

B7-H2/ICOSLG Antibody (599841) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
  Glu47-Lys279
  Accession # Q9JHJ8
• Specificity: Detects mouse B7-H2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-H2, rhB7-H3,
  rhB7-H3b, rhPD-L2, recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H3, rmB7-H4, rmPD-L2, or recombinant rat B7-2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: ICOSLG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H2/ICOSLG Antibody (599841) [Unconjugated]

• B7H2
• B7-H2
• B7H2B7 homolog 2
• B7-H2B7-related protein 1
• B7RP-1
• B7RP1B7-like protein Gl50
• B7RP-1LICOS
• CD275 antigen
• CD275
• GL50
• GL50transmembrane protein B7-H2 ICOS ligand
• ICOSL
• ICOS-L
• ICOSLG
• inducible T-cell co-stimulator ligand
• KIAA0653ICOS ligand

Background

B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-H2/ICOSLG Antibody (MAB158)(2)

Publications using MAB158

• Guillem Angulo, Jelena Zeleznjak, Pablo Martínez-Vicente, Joan Puñet-Ortiz, Hartmut Hengel, Martin Messerle et al. Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion eLife 2021-01-18 [PMID: 33459589]
• Guillem Angulo, Jelena Zeleznjak, Pablo Martínez-Vicente, Joan Puñet-Ortiz, Hartmut Hengel, Martin Messerle et al. Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion eLife 2021-01-18 [PMID: 33459589] (Immunocytochemistry, Human, Mouse)

Bioinformatics

• Gene Symbol: ICOSLG
• Uniprot:
  • Q9JHJ8()