B7-H2/ICOSLG Antibody (2D3)

Images

 
Immunohistochemistry-Paraffin: B7-H2/ICOSLG Antibody (2D3) [NBP1-39919] - Analysis of anti-ICOSLG / ICOSL antibody with human brain, cortex at dilution 1:50.
Flow Cytometry: B7-H2/ICOSLG Antibody (2D3) [NBP1-39919] - Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-39919
    • Availability
      Product Discontinued

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B7-H2/ICOSLG Antibody (2D3) Summary

Immunogen
Full-length human B7H2-mIg fusion protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ICOSLG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 ug/million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50
Application Notes
IHC: NBP1-39919 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for B7-H2/ICOSLG Antibody (2D3)

  • B7H2
  • B7-H2
  • B7H2B7 homolog 2
  • B7-H2B7-related protein 1
  • B7RP-1
  • B7RP1B7-like protein Gl50
  • B7RP-1LICOS
  • CD275 antigen
  • CD275
  • GL50
  • GL50transmembrane protein B7-H2 ICOS ligand
  • ICOSL
  • ICOS-L
  • ICOSLG
  • inducible T-cell co-stimulator ligand
  • KIAA0653ICOS ligand

Background

B7-RP1 is a 40 kD protein, also known as ICOS ligand, B7-H2, and B7h. It has recently been clustered as CD275. It is a member of the immunoglobulin receptor superfamily expressed on splenic B cells, dendritic cells and macrophages. The expression of this antigen is increased by TNF- alpha stimulation and is highly upregulated by local inflammation. B7RP1 binds to ICOS and co-stimulates T cell and B cell responses. Two isoforms of this protein have been reported to occur as a result of alternative splicing. These isoforms show differential tissue distribution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ICOSLG
Entrez
Uniprot