B7-H2/ICOSLG Antibody (136726) [Phycoerythrin]

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7-H2 PE-conjugated Monoclonal Antibody (Catalog # FAB165P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 136726
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

B7-H2/ICOSLG Antibody (136726) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human B7-H2
  Asp19-Ser258
  Accession # O75144
• Specificity: Detects human B7-H2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, or recombinant mouse B7-H2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICOSLG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H2/ICOSLG Antibody (136726) [Phycoerythrin]

• B7H2
• B7-H2
• B7H2B7 homolog 2
• B7-H2B7-related protein 1
• B7RP-1
• B7RP1B7-like protein Gl50
• B7RP-1LICOS
• CD275 antigen
• CD275
• GL50
• GL50transmembrane protein B7-H2 ICOS ligand
• ICOSL
• ICOS-L
• ICOSLG
• inducible T-cell co-stimulator ligand
• KIAA0653ICOS ligand

Background

Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with human and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7‑H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
2. Ling, V. et al. (2000) J. Immunol. 164:1653.
3. Wang, S. et al. (2000) Blood 96:2808.
4. Brodie, D. et al. (2000) Curr. Biol. 10:333.
5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
6. Swallow, M.M. et al. (1999) Immunity 11:423.
7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-H2/ICOSLG Antibody (FAB165P)(2)

Publications using FAB165P

• CL Gigliotti, C Dianzani, I Stoppa, C Monge, S Sutti, D Sblattero, C Puricelli, R Rolla, U Dianzani, E Boggio Differential Modulation of Human M1 and M2 Macrophage Activity by ICOS-Mediated ICOSL Triggering International Journal of Molecular Sciences, 2023-02-02;24(3):. 2023-02-02 [PMID: 36769276] (Flow Cytometry, Human)
• Dianzani C, Minelli R, Gigliotti C, Occhipinti S, Giovarelli M, Conti L, Boggio E, Shivakumar Y, Baldanzi G, Malacarne V, Orilieri E, Cappellano G, Fantozzi R, Sblattero D, Yagi J, Rojo J, Chiocchetti A, Dianzani U B7h triggering inhibits the migration of tumor cell lines. J Immunol, 2014-04-11;192(10):4921-31. 2014-04-11 [PMID: 24729612] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ICOSLG
• Uniprot:
  • O75144()