B7-2/CD86 Antibody

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The CH‑1 mouse B cell lymphoma cell line, which expresses B7‑1 and B7‑2 on the cell surface, co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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B7-2/CD86 Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse B7‑2/CD86
Extracellular domain
Specificity
Detects mouse B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), less than 15% cross-reactivity with recombinant human B7-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD86
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 2-5 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF-441-NA in the following applications:

Publications
Read Publications using
AF-441-NA in the following applications:

  • ICC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-2/CD86 Antibody

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86

Background

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co‑stimulatory pathways that regulate T‑ and B‑cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7‑2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7‑2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-2 is a 309 amino acid (aa) protein containing a putative 23 aa signal peptide, a 221 aa extracellular domain, a 21 aa transmembrane domain, and a 44 aa cytoplasmic domain. Mouse B7-2 and B7-1 share 28% amino acid identity. Mouse and human B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA‑4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-2/CD86 Antibody (AF-441-NA)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC, WB.


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ICC
(1)
WB
(1)
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Filter By Species
Mouse
(2)
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Review for B7-2/CD86 Antibody (AF-441-NA) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF-441-NA:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen B7-2/CD86 AF-441-NA
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reviewed by:
Guo Mengqi
IHC-Fr Mouse 02/06/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse aortic root
SpeciesMouse
LotUE011704A

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FAQs for B7-2/CD86 Antibody (AF-441-NA) (0)

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Secondary Antibodies

 

Isotype Controls

Additional B7-2/CD86 Products

Bioinformatics Tool for B7-2/CD86 Antibody (AF-441-NA)

Discover related pathways, diseases and genes to B7-2/CD86 Antibody (AF-441-NA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B7-2/CD86 Antibody (AF-441-NA)

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Pathways for B7-2/CD86 Antibody (AF-441-NA)

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PTMs for B7-2/CD86 Antibody (AF-441-NA)

Learn more about PTMs related to B7-2/CD86 Antibody (AF-441-NA).

Blogs on B7-2/CD86.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews

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5
1
4
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2
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1
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Guo Mengqi
02/06/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CD86
Entrez