Mouse B7-1/CD80 ELISA Kit (Colorimetric)

• Reactivity: Mu
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Supernatant, Tissue Lysate
• Standard Curve Range: 62.5 to 4000 pg/mL
• Sensitivity: <10 pg/mL

Mouse B7-1/CD80 ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. Cd80 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Cd80.
• Standard Curve Range: 62.5 to 4000 pg/mL
• Sensitivity: <10 pg/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CD80

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse B7-1/CD80 ELISA Kit (Colorimetric)

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for B7-1/CD80 ELISA Kit (KA0991)(2)

Publications using KA0991

• Reeves RH, Patch D, Sharpe AH et al. The costimulatory genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3. Mamm Genome. 1997-08-01 [PMID: 9250865]
• Selvakumar A, Mohanraj BK, Eddy RL et al. Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7. Immunogenetics. 1992-01-01 [PMID: 1377173]

FAQs for B7-1/CD80 ELISA Kit (KA0991). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CD80