B7-1/CD80 Antibody (MEM-233)

Product Discontinued

B7-1/CD80 Antibody (MEM-233) Summary

• Immunogen: Extracellular domain of human CD80 fused to human IgG1(Fc)
• Localization: Cell Membrane
• Specificity: The antibody MEM-233 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD80
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunoprecipitation 1:50
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for B7-1/CD80 Antibody (MEM-233)

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-1/CD80 Antibody (NB500-440)(2)

Publications using NB500-440

• Nikolaitchik OA, Galli A, Moore MD et al. Multiple Barriers to Recombination between Divergent HIV-1 Variants Revealed by a Dual-Marker Recombination Assay J Mol Biol 2011-02-03 [PMID: 21295586]
  
  Details:
  This citation used the Allophycocyanin version of this antibody.
• Nikolaitchik OA, Galli A, Moore MD et al. Multiple Barriers to Recombination between Divergent HIV-1 Variants Revealed by a Dual-Marker Recombination Assay J Mol Biol
  
  Details:
  This publication used the Allophycocyanin conjugated form of this antibody (Cat# NB110-81741).

Bioinformatics

• Gene Symbol: CD80
• Entrez:
  • 941(Human)