B7-1/CD80 Antibody (AP-MAB0713) - Azide and BSA Free

Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (AP-MAB0713) [NBP2-12178] - Formalin fixed and paraffin embedded mouse tumor section was subjected to IHC staining of CD80 using NBP2-12178 (Left panel: x 20; Right panel: x 40).

Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (AP-MAB0713) [NBP2-12178] - Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to IHC staining of CD80 using NBP2-12178. (Left panel: Red Pulp, Right panel: White Pulp).

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready
• Clone: AP-MAB0713
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

B7-1/CD80 Antibody (AP-MAB0713) - Azide and BSA Free Summary

• Description: Novus Biologicals Armenian Hamster B7-1/CD80 Antibody (AP-MAB0713) - Azide and BSA Free (NBP2-12178) is a monoclonal antibody validated for use in IHC and Flow. Anti-B7-1/CD80 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Mouse recombinant B7-1/CD80
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: CD80
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100-1:1000
  • Immunohistochemistry 1:100-300
  • Immunohistochemistry-Paraffin 1:100-1:300
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for B7-1/CD80 Antibody (AP-MAB0713) - Azide and BSA Free

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-1/CD80 Antibody (NBP2-12178)(1)

Publication using NBP2-12178

• Samuelson DR, de la Rua NM, Charles TP et al. Oral Immunization of Mice with Live Pneumocystis murina Protects against Pneumocystis Pneumonia. J. Immunol. 2016-03-15 [PMID: 26864029] (FLOW, Mouse)
  
  Details:
  This publication used the PCP conjugated form of this antibody (NBP2-25235PCP)

Bioinformatics

• Gene Symbol: CD80
• Uniprot:
  • Q00609()