B7-1/CD80 Antibody (16C10) [Biotin]

• Reactivity: Hu
• Applications: ELISA, B/N
• Clone: 16C10
• Clonality: Monoclonal
• Host: Human
• Conjugate: Biotin

B7-1/CD80 Antibody (16C10) [Biotin] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen.
• Specificity: This antibody binds human B7-1/CD80 and inhibits the B7.1:CD28 pathway.
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Human
• Gene: CD80
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for B7-1/CD80 Antibody (16C10) [Biotin]

• Activation B7-1 antigen
• B7
• B71
• B7-1
• BB1
• CD28LG1
• CD28LGB7-1 antigen)
• CD80 antigen
• CD80 molecule
• CD80
• costimulatory factor CD80
• costimulatory molecule variant IgV-CD80
• CTLA-4 counter-receptor B7.1
• T-lymphocyte activation antigen CD80

Background

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD80