B220/CD45R Antibody (RA3-6B2) [Allophycocyanin]

Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and A) Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) or B) RatIgG2AAPC-conjugated isotype control antibody (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: RA3-6B2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

B220/CD45R Antibody (RA3-6B2) [Allophycocyanin] Summary

• Immunogen: Abelson mouse leukemia virus-induced pre-B tumor cells
• Specificity: Detects mouse B220/CD45R.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PTPRC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B220/CD45R Antibody (RA3-6B2) [Allophycocyanin]

• B220
• CD45 antigen
• CD45R
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
5. Asensi, V.K. et al. (1989) Immunology 68:204.
6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B220/CD45R Antibody (FAB1217A)(1)

Publication using FAB1217A

• Notley CA, Brown MA, Wright GP, Ehrenstein MR Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells. J. Immunol., 2011-03-07;186(8):4967-72. 2011-03-07 [PMID: 21383247] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: PTPRC