B-Raf Antibody (RM8) - V600-E Mutation

Images

 
Western Blot: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein.
Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections.
Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue. Allele specific PCR validated to be ...read more
Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Staining of formalin fixed and paraffin embedded human cell line sections.
Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Staining of formalin fixed and paraffin embedded human cell line sections.
Immunohistochemistry-Paraffin: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue. Allelespecific PCR validated to be Positive for BRAF V600E.
ELISA: B-Raf Antibody (RM8) - V600-E Mutation [NBP2-61503] - Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or B-Raf wild type His-tag proteins, shows the specificity of NBP2-61503 to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
RM8
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

B-Raf Antibody (RM8) - V600-E Mutation Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
A peptide corresponding to B-Raf V600E mutant
Specificity
This antibody reacts to the B-Raf V600E mutant. No cross reactivity with wild type BRAF.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BRAF
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence 0.5-5 ug/ml
  • Immunohistochemistry 0.5-5 ug/mL
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
50% Glycerol/PBS with 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for B-Raf Antibody (RM8) - V600-E Mutation

  • 94 kDa B-raf protein
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
  • BRaf
  • B-Raf
  • BRAF1
  • B-RAF1
  • BRAF1MGC126806
  • EC 2.7.11.1
  • MGC138284
  • murine sarcoma viral (v-raf) oncogene homolog B1
  • NS7
  • p94
  • Proto-oncogene B-Raf
  • RAFB1
  • serine/threonine-protein kinase B-raf
  • v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BRAF