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B-Raf Antibody (OTI5A9) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
OTI5A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70260AF350
Alexa Fluor 405
NBP2-70260AF405
Alexa Fluor 532
NBP2-70260AF532
Alexa Fluor 594
NBP2-70260AF594
Alexa Fluor 647
NBP2-70260AF647
Alexa Fluor 700
NBP2-70260AF700
Alexa Fluor 750
NBP2-70260AF750
Allophycocyanin
NBP2-70260APC
Biotin
NBP2-70260B
DyLight 350
NBP2-70260UV
DyLight 405
NBP2-70260V
DyLight 488
NBP2-70260G
DyLight 550
NBP2-70260R
DyLight 594
NBP2-70260DL594
DyLight 650
NBP2-70260C
DyLight 680
NBP2-70260FR
DyLight 755
NBP2-70260IR
FITC
NBP2-70260F
HRP
NBP2-70260H
Janelia Fluor 549
NBP2-70260JF549
Janelia Fluor 646
NBP2-70260JF646
PE
NBP2-70260PE
PerCP
NBP2-70260PCP
Unconjugated
NBP1-47668
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47668
Azide and BSA Free
NBP2-70260
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B-Raf Antibody (OTI5A9) [Alexa Fluor® 488] Summary

Immunogen
Full length recombinant protein of human B-Raf (NP_004324) produced in HEK293T cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BRAF
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for B-Raf Antibody (OTI5A9) [Alexa Fluor® 488]

  • 94 kDa B-raf protein
  • B-Raf proto-oncogene serine/threonine-protein kinase (p94)
  • BRaf
  • B-Raf
  • BRAF1
  • B-RAF1
  • BRAF1MGC126806
  • EC 2.7.11.1
  • MGC138284
  • murine sarcoma viral (v-raf) oncogene homolog B1
  • NS7
  • p94
  • Proto-oncogene B-Raf
  • RAFB1
  • serine/threonine-protein kinase B-raf
  • v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.
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Western blot shows lysates of MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, L‑929 mouse fibroblast cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Raf‑1 Monoclonal Antibody (Catalog # MAB4540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Raf‑1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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PIK3CA Antibody
NBP2-19804
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NB200-106
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Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.
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CCDC6 Antibody
NBP1-85351
Western Blot: CCDC6 Antibody [NBP1-85351] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: CCDC6 Antibody [NBP1-85351] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
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Human Factor IX
ELISA Kit (Colorimetr ...
NBP2-60518
ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518] - Standard curve.
ELISA: Human Factor IX ELISA Kit (Colorimetric) [NBP2-60518] - Standard curve.
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TAS2R38 Antibody
NBP2-80532
TAS2R38 Antibody
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SNRPE Antibody
NBP2-94255
Western Blot: SNRPE Antibody [NBP2-94255] - Analysis of extracts of various cell lines, using SNRPE at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/Immunofluorescence: SNRPE Antibody [NBP2-94255] - Analysis of U2OS cells using SNRPE. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SNRPE Antibody [NBP2-94255] - Paraffin-embedded mouse stomach using SNRPE.
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for B-Raf Antibody (NBP2-70260AF488) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70260AF350
Alexa Fluor 405 NBP2-70260AF405
Alexa Fluor 488 NBP2-70260AF488
Alexa Fluor 532 NBP2-70260AF532
Alexa Fluor 594 NBP2-70260AF594
Alexa Fluor 647 NBP2-70260AF647
Alexa Fluor 700 NBP2-70260AF700
Alexa Fluor 750 NBP2-70260AF750
Allophycocyanin NBP2-70260APC
Biotin NBP2-70260B
DyLight 350 NBP2-70260UV
DyLight 405 NBP2-70260V
DyLight 488 NBP2-70260G
DyLight 550 NBP2-70260R
DyLight 594 NBP2-70260DL594
DyLight 650 NBP2-70260C
DyLight 680 NBP2-70260FR
DyLight 755 NBP2-70260IR
FITC NBP2-70260F
HRP NBP2-70260H
Janelia Fluor 549 NBP2-70260JF549
Janelia Fluor 646 NBP2-70260JF646
PE NBP2-70260PE
PerCP NBP2-70260PCP
Learn about Custom Antibody Labeling

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Bioinformatics Tool for B-Raf Antibody (NBP2-70260AF488)

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Blogs on B-Raf.

Check out the latest blog posts on B-Raf.
MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.
You can't be without me - SNF5
The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a...  Read full blog post.
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Bioinformatics

Gene Symbol BRAF