B-Raf Antibody (OTI5A9) - Azide and BSA Free

Western Blot: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antibody

Immunocytochemistry/Immunofluorescence: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY B Raf.

Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Staining of paraffin-embedded Human pancreas tissue using anti-B Raf mouse monoclonal antibody.

Flow Cytometry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-B Raf antibody, and then analyzed by flow cytometry.

Western Blot: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Analysis of extracts (35ug) from 9 different cell lines by using anti-B Raf monoclonal antibody.

Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - The images depict immunostaining of representative PTC cases harboring BRAF wild-type (A, B) and BRAF V600E mutation (C, D) proven by NGS; varying degrees of immunoreactivity for BRAF is shown both in the intranuclear inclusions (arrows) and in the cytopl

Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Staining of paraffin-embedded Human endometrium tissue using anti-B Raf mouse monoclonal antibody.

Immunohistochemistry: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - Staining of paraffin-embedded Human lung tissue using anti-B Raf mouse monoclonal antibody.

Immunoprecipitation: B-Raf Antibody (OTI5A9) - Azide and BSA Free [NBP2-70260] - (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: OTI5A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

B-Raf Antibody (OTI5A9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse B-Raf Antibody (OTI5A9) - Azide and BSA Free (NBP1-47668) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BRAF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:1000-2000
• Theoretical MW: 84.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for B-Raf Antibody (OTI5A9) - Azide and BSA Free

• 94 kDa B-raf protein
• B-Raf proto-oncogene serine/threonine-protein kinase (p94)
• BRaf
• B-Raf
• BRAF1
• B-RAF1
• BRAF1MGC126806
• EC 2.7.11.1
• MGC138284
• murine sarcoma viral (v-raf) oncogene homolog B1
• NS7
• p94
• Proto-oncogene B-Raf
• RAFB1
• serine/threonine-protein kinase B-raf
• v-raf murine sarcoma viral oncogene homolog B1FLJ95109

Background

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BRAF