Aurora A Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-90103] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & centrosome.

Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human duodenum shows weak to moderate cytoplasmic positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Aurora A Antibody [NBP1-90103] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in germinal center cells.

Analysis in human cell line SK-BR-3.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Aurora A Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYL
• Marker: Mitosis Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AURKA
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
• Control Peptide:

  Aurora A Protein (NBP1-90103PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Aurora A Antibody - BSA Free

• AIK
• AIKAurA
• ARK1
• ARK-1
• ARK1EC 2.7.11.1
• AURA
• AURKA
• Aurora A
• aurora kinase AhARK1
• aurora/IPL1-like kinase
• Aurora/IPL1-related kinase 1
• Aurora-related kinase 1
• Breast tumor-amplified kinase
• breast-tumor-amplified kinase
• BTAK
• BTAKAURORA2
• EC 2.7.11
• IPL1-related kinase
• serine/threonine kinase 6
• serine/threonine protein kinase 15
• Serine/threonine-protein kinase 15
• serine/threonine-protein kinase 6
• Serine/threonine-protein kinase aurora-A
• STK15
• STK15serine/threonine kinase 15
• STK6
• STK6MGC34538
• STK7

Background

The protein encoded by the Aurora A gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aurora A Antibody (NBP1-90103)(1)

Publication using NBP1-90103

• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).

Bioinformatics

• Gene Symbol: AURKA
• Uniprot:
  • A3KFJ0(Human)