ATRIP [p Ser239] Antibody - Azide Free

Western Blot: ATRIP [p Ser239] Antibody [NBP2-29537] - Western blot of mouse testis lysate showing specific immunolabeling of the ~86kd ATRIP protein phosphorylated at Ser239 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Mu, Hu, Bv, Pm, Sh
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

ATRIP [p Ser239] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 conjugated to KLH. Accession # Q8WXE1
• Modification: p Ser239
• Specificity: Specific for endogenous levels of the ~86 kDa ATRIP protein phosphorylated at Ser239. The antibody also recognizes a band at ~32 kDa.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
• Predicted Species: Human (100%), Sheep (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATRIP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for ATRIP [p Ser239] Antibody - Azide Free

• AGS1
• ATM and Rad3-related-interacting protein
• ATR interacting protein
• ATR-interacting protein
• ATRIP
• DKFZp762J2115
• FLJ12343
• MGC20625
• MGC21482
• MGC26740
• TREX1

Background

ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at ser239 (Venere et al, 2007).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATRIP