ATP6V1A Antibody - BSA Free

Immunoprecipitation:ATP6V1A AntibodyNBP3-29944] -Detection of human ATP6V1A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATP6V1A antibody NBP3-29944 (lot NBP3-29944-1) used for IP at 6 µg per reaction. ATP6V1A was also immunoprecipitated by rabbit anti-ATP6V1A.. For blotting immunoprecipitated ATP6V1A, NBP3-29944 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP6V1A Antibody - BSA Free Summary

• Immunogen: Between 450 and 500
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP6V1A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for ATP6V1A Antibody - BSA Free

• 70kD, isoform 1
• ATP6A1
• ATP6V1A
• ATP6V1A1
• ATP6V1A1ATPase, H+ transporting, lysosomal, subunit A1
• ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha polypeptide
• ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
• EC 3.6.3
• EC 3.6.3.14
• EC 7.1.2.2
• H(+)-transporting two-sector ATPase, subunit A
• H+-transporting ATPase chain A, vacuolar (VA68 type)
• HO68
• VA68
• vacuolar ATP synthase catalytic subunit A, ubiquitous isoform
• Vacuolar ATPase isoform VA68
• vacuolar proton pump alpha subunit 1
• Vacuolar proton pump subunit alpha
• V-ATPase 69 kDa subunit 1
• V-ATPase 69 kDa subunit
• V-ATPase A subunit 1
• V-ATPase subunit A
• Vma1
• VPP2
• V-type proton ATPase catalytic subunit A

Background

ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP6V1A