ATP6AP1 Antibody (3H11B12) - BSA Free

ELISA: ATP6AP1 Antibody (3H11B12) [NBP3-27175] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow Cytometry: ATP6AP1 Antibody (3H11B12) [NBP3-27175] - Analysis of Hela cells using ATP6AP1 mouse mAb (green) and negative control (red).

Western Blot: ATP6AP1 Antibody (3H11B12) [NBP3-27175] - Analysis using ATP6AP1 mAb against HEK293-6e (1) and human ATP6AP1 (AA: 51-151)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot: ATP6AP1 Antibody (3H11B12) [NBP3-27175] - Analysis using ATP6AP1 mAb against human ATP6AP1 (AA: 51-151) recombinant protein. (Expected MW is 37kDa)

Western Blot: ATP6AP1 Antibody (3H11B12) [NBP3-27175] - Analysis using ATP6AP1 mouse mAb against PC-3 (1), and Raw264.7 (2) cell lysate.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 3H11B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ATP6AP1 Antibody (3H11B12) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP6AP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ATP6AP1 Antibody (3H11B12) - BSA Free

• 16A
• Ac45
• ATP6IP1V-ATPase subunit S1
• ATP6S1ORF
• ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1
• ATPase, H+ transporting, lysosomal accessory protein 1
• H+ transporting, lysosomal interacting protein 1
• H-ATPase subunit
• MGC129781
• Protein XAP-3
• Vacuolar proton pump subunit S1
• VATPS1V-ATPase Ac45 subunit
• V-type proton ATPase subunit S1
• XAP-3
• XAP3V-ATPase S1 accessory protein

Background

ATP6AP1 encodes a component of a multisubunit enzyme (1 mDa MW) that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene is approximately 45 kD and may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP6AP1