ATP5A Antibody (JM110-04)

Western Blot: ATP5A Antibody (JM110-04) [NBP2-67170] - Western blot analysis of ATP5A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SKOV-3 cell lysate Lane 2: mouse heart tissue lysate Lane 3: MCF-7 cell lysate

Immunocytochemistry/Immunofluorescence: ATP5A Antibody (JM110-04) [NBP2-67170] - Staining ATP5A1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATP5A Antibody (JM110-04) [NBP2-67170] - Analysis of paraffin-embedded mouse liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.

Flow Cytometry: ATP5A Antibody (JM110-04) [NBP2-67170] - Analysis of Hela cells with ATP5A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ATP5A Antibody (JM110-04) [NBP2-67170] - Staining ATP5A1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ATP5A Antibody (JM110-04) [NBP2-67170] - Staining ATP5A1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ATP5A Antibody (JM110-04) [NBP2-67170] - Analysis of paraffin-embedded human liver tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ATP5A Antibody (JM110-04) [NBP2-67170] - Analysis of paraffin-embedded mouse heart muscle tissue using anti-ATP5A1 antibody. Counter stained with hematoxylin.

Product Discontinued

ATP5A Antibody (JM110-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ATP5A aa 191-234 / 553. (SwissProt: P25705 Human; SwissProt: Q03265 Mouse; SwissProt: P15999 Rat)
• Localization: Cell membrane, CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATP5F1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ATP5A Antibody (JM110-04)

• ATP synthase alpha chain, mitochondrial
• ATP synthase subunit alpha, mitochondrial
• ATP synthase, H+ transporting, mitochondrial F1 co
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle
• ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2
• ATP sythase (F1-ATPase) alpha subunit
• ATP5A
• ATP5A1
• ATP5AL2
• ATP5AMOM2
• ATPM
• ATPMATP synthase subunit alpha, mitochondrial
• cardiac muscle
• COXPD22
• EC 3.6.3
• EC 3.6.3.14
• epididymis secretory sperm binding protein Li 123m
• hATP1
• HEL-S-123m
• MC5DN4
• MC5DN4A
• MC5DN4B
• mitochondrial ATP synthetase, oligomycin-resistant
• MOM2
• OMR
• ORM
• ORMATP synthase alpha chain, mitochondrial

Background

ATP5A encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP5F1A