ATP Citrate Lyase Antibody (3A5) - BSA Free

Western Blot: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] - Positive Western Blot detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate. All lanes: ATP Citrate Lyase Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 121, 120, 92 kDa Observed band size: 120 kDa

Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] - Staining of HepG2 Cells with ATP Citrate Lyase Antibody (3A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: ATP Citrate Lyase Antibody (3A5) [NBP3-26530] - Overlay histogram showing Hela cells stained with ATP Citrate Lyase Antibody (3A5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3A5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATP Citrate Lyase Antibody (3A5) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human ATP Citrate Lyase [UniProt P53396]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACLY
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:20-1:200
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ATP Citrate Lyase Antibody (3A5) - BSA Free

• ACLEC 2.3.3.8
• ACLY
• ATP Citrate Lyase
• ATP citrate synthase
• ATP-citrate (pro-S-)-lyase
• ATP-citrate synthase
• ATPCL
• Citrate cleavage enzyme
• CLATP
• EC 2.3.3.8

Background

ATP citrate lyase (ACLY), also known as ATP citrate synthase, is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACLY and insulin to increase phosphorylation of ACLY (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACLY