ATOH8 Antibody - BSA Free

Western Blot: ATOH8 Antibody [NBP1-77347] - A-20 cell lysate with ATOH8 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: ATOH8 Antibody - BSA Free [NBP1-77347] - Immunohistochemistry of ATOH8 in mouse brain tissue with ATOH8 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ATOH8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATOH8 Antibody - BSA Free (NBP1-77347) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATOH8. The immunogen is located within the last 50 amino acids of ATOH8. Amino Acid Squence: TRTLQAEGRAKKRKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATOH8
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ATOH8 Peptide (NBP1-77347PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ATOH8 Antibody - BSA Free

• ATH6
• atonal homolog 6
• atonal homolog 8 (Drosophila)
• basic helix-loop-helix transcription factor 6
• BHLHA21
• bHLHa21basic helix loop helix transcription factor 6
• Class A basic helix-loop-helix protein 21
• FLJ14708
• FLJ38730
• HATH6
• Helix-loop-helix protein hATH-6
• protein atonal homolog 8

Background

Basic helix-loop-helix (bHLH) transcription factors play important roles in differentiation processes during embryonic development of vertebrates. ATOH8 (MATH6) is a tissue-restricted member of the atonal superfamily of bHLH transcription factors that exhibits 43-57% identity in the bHLH domain with other mammalian atonal paralogs including the NeuroD and Neurogenin factors. In the mouse, ATOH8 has been implicated in the specification and differentiation of neuronal cell lineages in the brain and may also participate in kidney development. Recent studies show that ATOH8 is a novel component of the pancreatic transcriptional network during embryonic development and suggest a potential role as a modulator of the differentiation program initiated by the pro-endocrine factor Neurog3. It is indispensable for early embryonic development, suggesting a more widespread function for this factor in tissue-specific differentiation processes that are dependent on class II bHLH genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATOH8
• Uniprot:
  • NP_116216(Human)