ATG16L1 Antibody - BSA Free

Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.

Western Blot: ATG16L1 Antibody [NBP2-15504] - Wild-type (WT) and ATG16L1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG16L1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ATG16L1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ATG16L1 Antibody - BSA Free (NBP2-15504) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ATG16L1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATG16L1. The exact sequence is proprietary.
• Localization: Cytoplasm; Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasm; Preautophagosomal structure membrane
• Predicted Species: Mouse (93%), Rat (94%), Porcine (93%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATG16L1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: ICC/IF-Assay dependent
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ATG16L1 Antibody - BSA Free

• APG16 autophagy 16-like (S. cerevisiae)
• APG16L beta
• APG16LFLJ10828
• APG16-like 1
• ATG16 autophagy related 16-like (S. cerevisiae)
• ATG16 autophagy related 16-like 1 (S. cerevisiae)
• ATG16A
• ATG16L
• autophagy-related protein 16-1
• FLJ00045
• FLJ10035
• FLJ22677
• IBD10
• WD repeat domain 30
• WDR30

Background

Autophagy is the major intracellular degradation system delivering cytoplasmic components to lysosomes, and it accounts for degradation of most long-lived proteins and some organelles. Cytoplasmic constituents, including organelles, are sequestered into double-membraned autophagosomes, which subsequently fuse with lysosomes. ATG16L1 is a component of a large protein complex essential for autophagy (Mizushima et al., 2003 [PubMed 12665549]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ATG16L1 Antibody (NBP2-15504)(1)

Publication using NBP2-15504

• Palanisamy k, Tsai TH, Yu TM et al. RNA-binding protein, human antigen R regulates hypoxia-induced autophagy by targeting ATG7/ATG16L1 expressions and autophagosome formation. J. Cell. Physiol. 2018-10-14 [PMID: 30317574] (WB, Human)

Bioinformatics

• Gene Symbol: ATG16L1
• Uniprot:
  • Q676U5(Human)