ATG16L1 Antibody (S03-4G8)

Western Blot: ATG16L1 Antibody (S03-4G8) [NBP3-19576] - Western blot detection of ATG16L1 in 3T3 cell lysates using NBP3-19576 (1:1000 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S03-4G8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

ATG16L1 Antibody (S03-4G8) Summary

• Description: Novus Biologicals Rabbit ATG16L1 Antibody (S03-4G8) (NBP3-19576) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human ATG16L1 (Uniprot # Q676U5)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATG16L1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000-1/2000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for ATG16L1 Antibody (S03-4G8)

• APG16 autophagy 16-like (S. cerevisiae)
• APG16L beta
• APG16LFLJ10828
• APG16-like 1
• ATG16 autophagy related 16-like (S. cerevisiae)
• ATG16 autophagy related 16-like 1 (S. cerevisiae)
• ATG16A
• ATG16L
• autophagy-related protein 16-1
• FLJ00045
• FLJ10035
• FLJ22677
• IBD10
• WD repeat domain 30
• WDR30

Background

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is essential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane. There are 5 isoforms of this protein (in human), with theoretical molecular weights ranging from ~19-68 kDa.

Mutations in the ATG16L1 proitein have been associated with Crohn's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATG16L1