ATG14 Antibody

Images

 
Western Blot: ATG14 Antibody [NBP2-36445] - DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target ...read more
Immunocytochemistry/ Immunofluorescence: ATG14 Antibody [NBP2-36445] - Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 ug/mL.
Western Blot: ATG14 Antibody [NBP2-36445] - Analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ATG14 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG14. The immunogen is located within amino acids 270 - 320 of ATG14. Amino Acid Squence: VEEKKTTQGPDMEQSNPA
Specificity
At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG14
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Western Blot 1-2 ug/ml
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-36445 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for ATG14 Antibody

  • ATG14 Autophagy Related 14 Homolog (S. Cerevisiae)
  • ATG14 Autophagy Related 14 Homolog
  • ATG14L
  • Autophagy Related 14
  • Autophagy-Related Protein 14-Like Protein
  • Barkor
  • Beclin 1-Associated Autophagy-Related Key Regulator
  • Beclin 1-interacting protein
  • KIAA0831

Background

ATG14 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF

Publications for ATG14 Antibody (NBP2-36445)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATG14 Antibody (NBP2-36445) (0)

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Secondary Antibodies

 

Isotype Controls

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Additional ATG14 Products

Array NBP2-36445

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Diseases for ATG14 Antibody (NBP2-36445)

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PTMs for ATG14 Antibody (NBP2-36445)

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Blogs on ATG14.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation ...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

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Bioinformatics

Gene Symbol ATG14