AMBRA1 Antibody


Western Blot: AMBRA1 Antibody [NBP1-07124] - WB analysis of AMBRA1 in mouse liver cell lysate.
Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody [NBP1-07124] - AMBRA1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 more
Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse brain using DAB with hematoxylin counterstain.
Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse epidermis using DAB with hematoxylin counterstain.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
1.0 mg/ml

AMBRA1 Antibody Summary

A synthetic peptide derived from mouse activating molecule in Beclin 1-regulated/AMBRA1 protein sequence [amino acids range 1175-1250] [UniProt # A2AH22]
Cytoplasmic vesicle, autophagosome.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This AMBRA1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~ 142 kDa representing AMBRA1. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed compartmentalized in cytoplasmic vesicles of mouse brain and skin tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Read Publications using
NBP1-07124 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Human, Mouse, and Rat.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for AMBRA1 Antibody

  • activating molecule in BECN1-regulated autophagy protein 1
  • autophagy/beclin-1 regulator 1
  • DCAF3
  • DDB1 and CUL4 associated factor 3
  • FLJ20294
  • KIAA1736activating molecule in beclin-1-regulated autophagy
  • MGC33725
  • WD repeat domain 94
  • WDR94


AMBRA1 (Activating molecule in Beclin1-regulated autophagy protein 1, Kiaa1736, DCAF3) is a regulator of autophagy and neural development in embryogenesis. Ambra1 controls autophagy in the central nervous system by activating class III PtdIns3k complexes that are required for phagophore nucleation. Beclin1 and Vps34 interaction are promoted by AMBRA1, and help form this complex. The loss of AMBRA1 causes upregulated apoptosis and accumulation of ubiquitinated proteins, which finally proves lethal. It was recently discovered that AMBRA1 interacts with Parkin after prolonged mitochondrial depolarization, thus triggering mitophagy (PMID 21921694). AMBRA1 has 7 known isoforms, and numerous WD40 domains.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMBRA1 Antibody (NBP1-07124)(3)

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Product General Protocols

View specific protocols for AMBRA1 Antibody (NBP1-07124): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AMBRA1 Antibody (NBP1-07124). (Showing 1 - 1 of 1 FAQs).

  1. I would like to use the antibody (Cat.No. NBP1-07124) with chicken samples. Could you align the immunogen of this antibody to check, if any or all of the predicted chicken isoforms show homology with the Immunizing peptide?
    • We ran alignment of chicken's AMBRA1 sequence with the immunogen of NBP1-07124, and found that this antibody will not be able to detect this target in chicken samples.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-07124AF405
Alexa Fluor 488 Labeled NBP1-07124AF488
Alexa Fluor 647 Labeled NBP1-07124AF647
Alexa Fluor 700 Labeled NBP1-07124AF700
Biotin Labeled NBP1-07124B
DyLight 405 Labeled NBP1-07124V
DyLight 488 Labeled NBP1-07124G
DyLight 550 Labeled NBP1-07124R
DyLight 650 Labeled NBP1-07124C
FITC Labeled NBP1-07124F
HRP Labeled NBP1-07124H

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Blogs on AMBRA1.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

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Gene Symbol AMBRA1