Western Blot: AMBRA1 Antibody [NBP1-07124] - WB analysis of AMBRA1 in mouse liver cell lysate.
Immunocytochemistry/ Immunofluorescence: AMBRA1 Antibody [NBP1-07124] - AMBRA1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse brain using DAB with hematoxylin counterstain.
Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse epidermis using DAB with hematoxylin counterstain.
This AMBRA1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~ 142 kDa representing AMBRA1. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed compartmentalized in cytoplasmic vesicles of mouse brain and skin tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Read Publications using NBP1-07124 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for AMBRA1 Antibody
activating molecule in BECN1-regulated autophagy protein 1
autophagy/beclin-1 regulator 1
DDB1 and CUL4 associated factor 3
KIAA1736activating molecule in beclin-1-regulated autophagy
WD repeat domain 94
AMBRA1 (Activating molecule in Beclin1-regulated autophagy protein 1, Kiaa1736, DCAF3) is a regulator of autophagy and neural development in embryogenesis. Ambra1 controls autophagy in the central nervous system by activating class III PtdIns3k complexes that are required for phagophore nucleation. Beclin1 and Vps34 interaction are promoted by AMBRA1, and help form this complex. The loss of AMBRA1 causes upregulated apoptosis and accumulation of ubiquitinated proteins, which finally proves lethal. It was recently discovered that AMBRA1 interacts with Parkin after prolonged mitochondrial depolarization, thus triggering mitophagy (PMID 21921694). AMBRA1 has 7 known isoforms, and numerous WD40 domains.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for AMBRA1 Antibody (NBP1-07124). (Showing 1 - 1 of 1 FAQs).
I would like to use the antibody (Cat.No. NBP1-07124) with chicken samples. Could you align the immunogen of this antibody to check, if any or all of the predicted chicken isoforms show homology with the Immunizing peptide?
We ran alignment of chicken's AMBRA1 sequence with the immunogen of NBP1-07124, and found that this antibody will not be able to detect this target in chicken samples.
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