AMBRA1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: AMBRA1 Antibody [NBP1-07124] - Double labeling for Ambra1 (green) and PV (red) in the CA1 from a WT female; sections were DAPI-counterstained (blue) for neuronal nuclei (scale bar: 50 um). The higher magnification image (scale bar, 5 um) from the stratum pyramidale shows a PV neuron (right; merge in orange) and an unidentified neuron (left), both expressing Ambra1. The DAPI signal is omitted for clarity and neuron borders are indicated by a dash line (n=3 mice; three sections per animal). Image collected and cropped by CiteAb from the following publication (link.springer.com/article/10.1007/s12035-018-0911-5), licensed under a CC-BY license.

Western Blot: AMBRA1 Antibody [NBP1-07124] - WB analysis of AMBRA1 in mouse liver cell lysate.

Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse brain using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: AMBRA1 Antibody [NBP1-07124] - AMBRA1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: AMBRA1 Antibody [NBP1-07124] - IHC analysis of AMBRA1 in mouse epidermis using DAB with hematoxylin counterstain.

The protein levels of AMBRA1, LC3 and SQSTM1 were analyzed by western blot from fresh tissue samples. Tubulin was also detected as the control of sample loading. Representative western blots were shown. N normal tissue, T PCa tissue.

Western Blot: AMBRA1 Antibody - BSA Free [NBP1-07124] - a Immunohistochemical results showing the prevalence of AMBRA1 (left) & SQSTM1 (right) based on a semi-quantitative total score. Frequency distribution of the protein is reported according to the Gleason grade classification (GL6–GL8). A trend of AMBRA1 high score values correlation with the higher grade of Gleason score is visible. As opposite the levels of SQSTM1 positivity appeared higher in tumors with a lower Gleason score (Gleason 6). b The protein levels of AMBRA1, LC3 & SQSTM1 were analyzed by western blot from fresh tissue samples. Tubulin was also detected as the control of sample loading. Representative western blots were shown. N normal tissue, T PCa tissue. c mRNA expression of AMBRA1 & SQSTM1 clearly shows that a significant upregulation of these genes occurs in PCa in comparison with BPH. * P < 0.01; ° P < 0.05 Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s10495-015-1176-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

AMBRA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AMBRA1 Antibody - BSA Free (NBP1-07124) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-AMBRA1 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide derived from mouse activating molecule in Beclin 1-regulated/AMBRA1 protein sequence [amino acids range 1175-1250] [UniProt # A2AH22]
• Localization: Cytoplasmic vesicle, autophagosome.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMBRA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 1:1000
• Application Notes: This AMBRA1 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~ 142 kDa representing AMBRA1. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed compartmentalized in cytoplasmic vesicles of mouse brain and skin tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AMBRA1 Antibody - BSA Free

• activating molecule in BECN1-regulated autophagy protein 1
• autophagy/beclin-1 regulator 1
• DCAF3
• DDB1 and CUL4 associated factor 3
• FLJ20294
• KIAA1736activating molecule in beclin-1-regulated autophagy
• MGC33725
• WD repeat domain 94
• WDR94

Background

AMBRA1 (Activating molecule in Beclin1-regulated autophagy protein 1, Kiaa1736, DCAF3) is a regulator of autophagy and neural development in embryogenesis. Ambra1 controls autophagy in the central nervous system by activating class III PtdIns3k complexes that are required for phagophore nucleation. Beclin1 and Vps34 interaction are promoted by AMBRA1, and help form this complex. The loss of AMBRA1 causes upregulated apoptosis and accumulation of ubiquitinated proteins, which finally proves lethal. It was recently discovered that AMBRA1 interacts with Parkin after prolonged mitochondrial depolarization, thus triggering mitophagy (PMID 21921694). AMBRA1 has 7 known isoforms, and numerous WD40 domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMBRA1 Antibody (NBP1-07124)(6)

Publications using NBP1-07124

• RamIrez-Pardo I, Villarejo-Zori B, JimEnez-Loygorri JI et al. Ambra1 haploinsufficiency in CD1 mice results in metabolic alterations and exacerbates age-associated retinal degeneration Autophagy 2022-07-24 [PMID: 35875981] (ICC/IF, Mouse)
  
  Details:
  Dilutions: 1:200
• Li L, Zuo X, Liu D Et al. Plasma exosomal RNAs has potential as both clinical biomarkers and therapeutic targets of dermatomyositis Rheumatology (Oxford, England) 2021-10-26 [PMID: 34698812] (WB, IM)
• Antonioli M, Pagni B, Vescovo T et al. HPV sensitizes OPSCC cells to cisplatin-induced apoptosis by inhibiting autophagy through E7-mediated degradation of AMBRA1 Autophagy 2020-11-23 [PMID: 33172332] (Western Blot)
• Nobili A, Krashia P, Cordella A et al. Ambra1 Shapes Hippocampal Inhibition/Excitation Balance: Role in Neurodevelopmental Disorders. Mol. Neurobiol. 2018-02-27 [PMID: 29488136] (ICC/IF, Mouse)
• Ugun-Klusek A, Tatham MH, Elkharaz J et al. Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway Cell Death Dis 2017-01-05 [PMID: 28055010] (IF/IHC, Mouse)
• Falasca L, Torino F, Marconi M et al. AMBRA1 and SQSTM1 expression pattern in prostate cancer. Apoptosis 2015-12-01 [PMID: 26423274] (WB)

FAQs for AMBRA1 Antibody (NBP1-07124). (Showing 1 - 1 of 1 FAQs).

1. I would like to use the antibody (Cat.No. NBP1-07124) with chicken samples. Could you align the immunogen of this antibody to check, if any or all of the predicted chicken isoforms show homology with the Immunizing peptide?
   • We ran alignment of chicken's AMBRA1 sequence with the immunogen of NBP1-07124, and found that this antibody will not be able to detect this target in chicken samples.

Bioinformatics

• Gene Symbol: AMBRA1
• Entrez:
  • 55626(Human)
  • 228361(Mouse)
  • 59319(Rat)
• Uniprot:
  • A2AH22(Mouse)