![Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against HEK293 (1) and ATG14 (AA: 43-303)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024920238.jpg%3Fv=1666701343 "Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against HEK293 (1) and ATG14 (AA: 43-303)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | 1F7C10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Mouse ATG14 Antibody (1F7C10) - BSA Free (NBP3-27119) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Purified recombinant fragment of human ATG14 (AA: 43-303) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ATG14 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 55.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ATG14 |
![ELISA: ATG14 Antibody (1F7C10) [NBP3-27119] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024916246.jpg%3Fv=1666701343 "ELISA: ATG14 Antibody (1F7C10) [NBP3-27119] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)")
![Immunohistochemistry: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis of paraffin-embedded testis tissues using ATG14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-104202491703.jpg%3Fv=1666701343 "Immunohistochemistry: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis of paraffin-embedded testis tissues using ATG14 mouse mAb with DAB staining.")
![Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against human ATG14 (AA: 43-303) recombinant protein. (Expected MW is 56.4 kDa)](http://images.novusbio.com/fullsize/nbp3-27119_mouse-atg14-mab-1f7c10-bsa-free-1042024915810.jpg%3Fv=1666701343 "Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against human ATG14 (AA: 43-303) recombinant protein. (Expected MW is 56.4 kDa)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")