ATF3 Antibody

Images

 
Western Blot: ATF3 Antibody [NBP1-02935] - Western blot analysis of ATF3 on UV treated NIH/3T3 cell extracts using NBP1-02935.
Immunohistochemistry: ATF3 Antibody [NBP1-02935] - Analysis of ATF3 in human prostate using Fast Red with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1.27 mg/ml

ATF3 Antibody Summary

Immunogen
A synthetic peptide corresponding to a region of human ATF3 (within residues 100-150). [UniProt# P18847]
Clonality
Polyclonal
Host
Rabbit
Gene
ATF3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.27 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
This ATF3 antibody is useful for Western Blot, ELISA and Immunohistochemistry paraffin embedded sections.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-02935 in the following applications:

Reactivity Notes

Human, mouse and rat. Predicted to react with most mammalian species based on sequence homology.

Alternate Names for ATF3 Antibody

  • activating transcription factor 3cAMP-dependent transcription factor ATF-3
  • ATF3deltaZip2
  • ATF3deltaZip2c
  • ATF3deltaZip3
  • cyclic AMP-dependent transcription factor ATF-3

Background

ATF3 (activating transcription factor 3) belongs to ATF/CREB family of transcription factors and it binds Cre 5'-GTGACGT[AC][AG]-3' a sequence which is present in several cellular as well as viral promoters. ATF3 exerts its repressive effects on transcription from promoters with ATF sites by stabilizing the binding of inhibitory cofactors of target promoters. Expressed by various cell types with nuclear localization, ATF3 expression gets upregulated by a variety of extracellular stimuli such as cytokines, chemokines, growth factors, hormones, hypoxia, DNA damage, ER stress and starvation. Originally considered a stress-response gene, ATF3 now has emerged as a factor implicated in general adaptive responses such as environmental, emotional and nutritional alterations. Its disorders have been associated with inflammatory and immune diseases as well as cancer, and unlike other ATF/CREB family members, ATF3 is emerging as a factor involved in protection against malignant cells and invading pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB8930
Species: Hu
Applications: WB, Simple Western, ICC
NB110-75039
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-1335
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
MAB1776
Species: Hu, Mu, Rt
Applications: WB
MAB1489
Species: Hu
Applications: WB
AF2818
Species: Hu
Applications: WB, ICC
NBP1-47842
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
NB100-410
Species: Hu, Mu
Applications: WB, IP
NBP1-85457
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00000468-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
NB100-56530
Species: Hu
Applications: WB
NBP1-89544
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-32822
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
MAB5526
Species: Hu, Mu
Applications: WB

Publications for ATF3 Antibody (NBP1-02935)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for ATF3 Antibody (NBP1-02935) (0)

There are no reviews for ATF3 Antibody (NBP1-02935). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

View specific protocols for ATF3 Antibody (NBP1-02935): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATF3 Antibody (NBP1-02935). (Showing 1 - 1 of 1 FAQs).

  1. We have a customer who is interested in the following antibodies: NB600-904 c-Fos ; NBP1-02935 ATF3. She is not sure which ones would be suitable for her studies. Her message is as follows: I have a new list of antibodies I would like you to check for me. We need them for paraformaldehyde perfused mice, the DRGs (dorsal root ganglia) cut by frozen section. So not paraffin processed. Would Novus be able to comment on the suitability of these antibodies for our tissues. I would also be grateful if they could make other suggestions if they have other more suitable antibodies or comment on which of the two Il1b antibodies would be the better. I am hoping you are able to help us with our customer.
    • While NBP1-02935 has not been tested on frozen sections, none of our other ATF3 antibodies have been tested either. NBP1-02935 works fine in IHC on paraffin embedded tissues, so should be suitable for use on frozen sections as well, although we can not guarantee that. Generally an antibody that is suitable for paraffin-embedded sections should work on frozen sections as well, however, we have seen evidence that this is not always true. This is why we make a distinction. The customer is more than welcome to try an IHC-P guaranteed antibody on frozen sections if they would like since it will most likely work, however, we can not guarantee it for this use. NB110-61652 is sheep polyclonal, not NB600-904 and once again, NB600-904 isn't guaranteed for use in frozen sections but the customer can certainly give it a try. I recommended NB110-75039 since it does have a frozen tissue guarantee. NB110-75039 and NB110-61652 are both guaranteed to detect c-Fos on frozen sections. I would recommend NB110-75039 since we do have images for this product and it is a rabbit polyclonal, so it is much more likely the customer would have an anti-rabbit secondary available rather than an anti-sheep secondary.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-02935AF405
Alexa Fluor 488 Labeled NBP1-02935AF488
Alexa Fluor 647 Labeled NBP1-02935AF647
Alexa Fluor 700 Labeled NBP1-02935AF700
Biotin Labeled NBP1-02935B
DyLight 405 Labeled NBP1-02935V
DyLight 488 Labeled NBP1-02935G
DyLight 550 Labeled NBP1-02935R
DyLight 650 Labeled NBP1-02935C
FITC Labeled NBP1-02935F
HRP Labeled NBP1-02935H

Additional ATF3 Antibody Products

Related Products by Gene

Bioinformatics Tool for ATF3 Antibody (NBP1-02935)

Discover related pathways, diseases and genes to ATF3 Antibody (NBP1-02935). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ATF3 Antibody (NBP1-02935)

Discover more about diseases related to ATF3 Antibody (NBP1-02935).
 

Pathways for ATF3 Antibody (NBP1-02935)

View related products by pathway.

PTMs for ATF3 Antibody (NBP1-02935)

Learn more about PTMs related to ATF3 Antibody (NBP1-02935).
 

Research Areas for ATF3 Antibody (NBP1-02935)

Find related products by research area.

Blogs on ATF3.

Multifaceted Roles of Matrix Metalloproteinase-2 (MMP2) in Normal and Disease State
MMP2 is a 72 kDa enzymatic protein and it belongs to matrix metalloproteinases (MMPs), a heterogenous family of zinc/calcium-dependent TIMPs (tissue inhibitors of matrix metalloproteinases) regulated matrix-degrading endopeptidases which are class...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol ATF3
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-02935 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought