ATF3 Antibody (S08-6H6)

Western Blot: ATF3 Antibody (S08-6H6) [NBP3-15001] - Western blot detection of ATF3 in A549, MCF-7, U251, HL-60 cell lysates using ATF3 Rabbit mAb (1:1000 diluted). Predicted band size: 21kDa. Observed band size: 21kDa.

• Reactivity: Hu
• Applications: WB
• Clone: S08-6H6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ATF3 Antibody (S08-6H6) Summary

• Description: Novus Biologicals Rabbit ATF3 Antibody (S08-6H6) (NBP3-15001) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human ATF3 (Uniprot#: P18847)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATF3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for ATF3 Antibody (S08-6H6)

• activating transcription factor 3cAMP-dependent transcription factor ATF-3
• ATF3deltaZip2
• ATF3deltaZip2c
• ATF3deltaZip3
• cyclic AMP-dependent transcription factor ATF-3

Background

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF3