Ataxin-3 Antibody

Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250.Blue: Hoechst 33342 staining.

Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:1000

Western Blot: Ataxin-3 Antibody [NBP1-32083] - Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human Ataxin 3. 10 % SDS-PAGEAtaxin 3 antibody dilution: 1:20000

Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.

• Reactivity: Hu, Bv, Sh
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ataxin-3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ataxin 3. The exact sequence is proprietary.
• Localization: Nucleus matrix
• Predicted Species: Sheep (92%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATXN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (80%), Rat (82%), Pig (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0) and 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Ataxin-3 Antibody

• AT3
• ataxin 3
• Ataxin3
• Ataxin-3
• ATX3AT3
• ATXN3
• EC 3.4.19.12
• EC 3.4.22
• JOS
• Machado-Joseph disease protein 1
• MJD1
• MJDolivopontocerebellar ataxia3, autosomal dominant, ataxin 3)
• olivopontocerebellar ataxia 3
• SCA3
• Spinocerebellar ataxia type 3 protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATXN3
• Entrez:
  • 4287(Human)
  • 110616(Mouse)
  • 60331(Rat)