| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12686-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92611-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99131-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-93456-25ul | |||
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0011.jpg "Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32083-img0008.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Various whole cell extracts (30 ug) were separated by 10 % SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:1000](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0006.jpg)
![Western Blot: Ataxin-3 Antibody [NBP1-32083] - Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human Ataxin 3. 10 % SDS-PAGE Ataxin 3 antibody dilution: 1:20000](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP1-32083-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP1-32083] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32083-img0007.jpg)
![Western Blot: Ataxin-3 Antibody [NBP3-12686] - Lane A: U-87MG Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP3-12686-img0001.jpg "Western Blot: Ataxin-3 Antibody [NBP3-12686] - Lane A: U-87MG Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:42 kDa")
![Immunohistochemistry-Paraffin: Ataxin-3 Antibody [NBP3-12686] - Staining of human ATXN3 in human testis with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunohistochemistry-Paraffin-NBP3-12686-img0002.jpg)
![Immunoprecipitation: Ataxin-3 Antibody [NBP3-12686] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-ATXN3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATXN3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size :50 kDa](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunoprecipitation-NBP3-12686-img0003.jpg)
![Western Blot: Ataxin-3 Antibody [NBP2-92611] - Analysis of extracts of various cell lines, using Ataxin-3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP2-92611-img0002.jpg "Western Blot: Ataxin-3 Antibody [NBP2-92611] - Analysis of extracts of various cell lines, using Ataxin-3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: Ataxin-3 Antibody [NBP2-92611] - Analysis of HeLa cells using Ataxin-3 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92611-img0001.jpg)
![Western Blot: Ataxin-3 Antibody [NBP2-87047] - Lanes: Lane 1: 2ug hATX3 (isoform2); Josephin domain(1-182). Lane 2: 2ug hATX3 (isoform2); C-terminal truncated Atx3 (1-264). Lane 3: 2ug hATX3 (isoform2). Lane 4: 2ug other protein. Primary Antibody Dilution: 1:10,000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:20,000. Gene Name: ATXN3. Submitted by: Dr. Maria Macedo, Instituto de Biologia Molecular e Celular.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP2-87047-img0002.jpg "Western Blot: Ataxin-3 Antibody [NBP2-87047] - Lanes: Lane 1: 2ug hATX3 (isoform2); Josephin domain(1-182). Lane 2: 2ug hATX3 (isoform2); C-terminal truncated Atx3 (1-264). Lane 3: 2ug hATX3 (isoform2). Lane 4: 2ug other protein. Primary Antibody Dilution: 1:10,000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:20,000. Gene Name: ATXN3. Submitted by: Dr. Maria Macedo, Instituto de Biologia Molecular e Celular.")
![Western Blot: Ataxin-3 Antibody [NBP2-87047] - WB Suggested Anti-ATXN3 Antibody Titration: 0.2-1 ug/ml. Positive Control: 293T cell lysate](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-Western-Blot-NBP2-87047-img0001.jpg)
![Western Blot: Ataxin-3 Antibody (S04-1I8) [NBP3-19834] - Western blot detection of Ataxin-3 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19834 (1:1000 diluted). Predicted band size: 42kDa. Observed band size: 42kDa.](http://images.novusbio.com/fullsize/Ataxin-3-Antibody-S04-1I8-Western-Blot-NBP3-19834-img0001.jpg "Western Blot: Ataxin-3 Antibody (S04-1I8) [NBP3-19834] - Western blot detection of Ataxin-3 in Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-19834 (1:1000 diluted). Predicted band size: 42kDa. Observed band size: 42kDa.")
![Western Blot: Ataxin 7-Like 3 Antibody [NBP2-99131] - Lane A: Jurkat Whole Cell LysateLane B: Raji Whole Cell LysateLane C: K562 Whole Cell LysateLane D: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:39 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Ataxin-7-Like-3-Antibody-Western-Blot-NBP2-99131-img0001.jpg "Western Blot: Ataxin 7-Like 3 Antibody [NBP2-99131] - Lane A: Jurkat Whole Cell LysateLane B: Raji Whole Cell LysateLane C: K562 Whole Cell LysateLane D: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:39 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Ataxin 7-Like 3 Antibody [NBP2-99131] - Lane A:0.5 mg Raji Whole Cell LysateLane B:0.5 mg K562 Whole Cell LysateLane C:0.5 mg THP-1 Whole Cell Lysate4 uL anti-ATXN7L3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATXN7L3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 45 kDa](http://images.novusbio.com/fullsize/Ataxin-7-Like-3-Antibody-Immunoprecipitation-NBP2-99131-img0002.jpg)
![Western Blot:[NBP3-30701]-Detection of human ATXN7L3 by western blot. Samples: Whole cell lysate from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATXN7L3 antibody used for WB at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30701_rabbit-ataxin-7-like-3-pab-23120251225646.jpg "Western Blot:[NBP3-30701]-Detection of human ATXN7L3 by western blot. Samples: Whole cell lysate from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATXN7L3 antibody used for WB at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Ataxin 7-Like 3 Antibody [NBP2-82691] - WB Suggested Anti-ATXN7L3 Antibody. Titration: 1.0 ug/ml. Positive Control: 721_B Whole Cell](http://images.novusbio.com/fullsize/Ataxin-7-Like-3-Antibody-Western-Blot-NBP2-82691-img0001.jpg "Western Blot: Ataxin 7-Like 3 Antibody [NBP2-82691] - WB Suggested Anti-ATXN7L3 Antibody. Titration: 1.0 ug/ml. Positive Control: 721_B Whole Cell")
![Immunohistochemistry: ATXN3L Antibody [NBP1-93456] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATXN3L-Antibody-Immunohistochemistry-NBP1-93456-img0003.jpg "Immunohistochemistry: ATXN3L Antibody [NBP1-93456] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.")