Asparagine synthetase Antibody (S05-9E5)

Western Blot: Asparagine synthetase Antibody (S05-9E5) [NBP3-19507] - Western blot detection of Asparagine synthetase in K562, Hela cell lysates using NBP3-19507 (1:1000 diluted). Predicted band size: 64kDa. Observed band size: 64kDa.

Immunohistochemistry-Paraffin: Asparagine synthetase Antibody (S05-9E5) [NBP3-19507] - Immunohistochemistry of Asparagine synthetase in paraffin-embedded Human tonsil using NBP3-19507 at dilution 1/100

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S05-9E5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Asparagine synthetase Antibody (S05-9E5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Asparagine synthetase (Uniprot # P08243)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ASNS
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Asparagine synthetase Antibody (S05-9E5)

• ASNS
• ASNSD
• asparagine synthetase (glutamine-hydrolyzing)
• asparagine synthetase [glutamine-hydrolyzing]
• asparagine synthetase
• Cell cycle control protein TS11
• EC 6.3.5.4
• Glutamine Dependent Asparagine Synthetase
• Glutamine-dependent asparagine synthetase
• TS11 Cell Cycle Control Protein
• TS11

Background

Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASNS