ASPA Antibody - BSA Free

Western Blot: ASPA Antibody [NBP2-15491] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ASPA antibody diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: ASPA Antibody [NBP2-15491] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Western Blot: ASPA Antibody [NBP2-15491] - Sample (20 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE gel, diluted at 1:10000.

• Reactivity: Hu, Mu, Po, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ASPA Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ASPA Antibody - BSA Free (NBP2-15491) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human ASPA Recombinant protein.
• Localization: Cytoplasm; Nucleus
• Predicted Species: Porcine (92%), Rhesus Macaque (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASPA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Western Blot 1:5000-1:20000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (85%), Rat (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ASPA Antibody - BSA Free

• ACY-2
• ACY2aminoacylase 2
• Aminoacylase-2
• ASPaminoacylase-2
• aspartoacylase (aminoacylase 2, Canavan disease)
• aspartoacylase
• EC 3.5.1.15

Background

This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ASPA
• Uniprot:
  • P45381(Human)