ASH2L Antibody - BSA Free

Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.

Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-281] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Chromatin Immunoprecipitation: ASH2L Antibody [NB600-281] - ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-281 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products abeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ASH2L Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 628) of human Absent, Small, or Homeotic-Like 2 using the numbering given in Swiss-Prot entry Q9UBL3 (GeneID 9070).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASH2L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1-5 ug
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:20000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  ASH2L Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ASH2L Antibody - BSA Free

• ash2 (absent, small, or homeotic)-like (Drosophila)
• ASH2
• ASH2L1ash2 (absent, small, or homeotic, Drosophila, homolog)-like
• ASH2L2ASH2-like protein
• Bre2
• set1/Ash2 histone methyltransferase complex subunit ASH2

Background

The ash2 gene is a member of the trithorax group of genes whose products function to maintain active transcription of homeotic selector genes. Mutations in ash2 cause the homeotic transformations expected for a gene in this group but, in addition, cause a variety of pattern formation defects that are not necessarily expected. The ash2 gene is located in cytogenetic region 96A17-19 flanked by slowpoke and tolloid and is included in a cosmid that contains part of slowpoke. Ash2, the product of the Drosophila trxG gene absent, small, or homeotic discs 2 (ash2) is a component of a 500-kD complex. ASH2 binds directly to Skittles (Sktl), a predicted phosphatidylinositol 4-phosphate 5-kinase, and the association of these proteins is functionally significant. Histone H1 hyperphosphorylation is dramatically increased in both ash2 and sktl mutant polytene chromosomes. These results suggest that Ash2 maintains active transcription by binding a producer of nuclear phosphoinositides and downregulating histone H1 hyperphosphorylation. Both Ash2 and Sktl accumulate on polytene chromosomes and in the nucleolus. These results suggest that their physical association and functions involve chromatin and, perhaps, ribosomal DNA transcription. Ash2 accumulates normally on sktl mutant chromosomes while Sktl does not accumulate on ash2 mutant chromosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ASH2L Antibody (NB600-281)(1)

Publication using NB600-281

• Bieluszewska A, Weglewska M, Bieluszewski T et al. PKA-binding domain of AKAP8 is essential for direct interaction with DPY30 protein FEBS J. 2017-12-30 [PMID: 29288530] (WB, Human)

Bioinformatics

• Gene Symbol: ASH2L
• Entrez:
  • 9070(Human)
• Uniprot:
  • Q9UBL3(Human)