| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61685-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88858-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61686-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18668-25ul | |||
![Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody NB600-250 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-250-img0015.jpg "Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody NB600-250 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-250] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ASH2used at a dilution of 1:1,000 ( 1ug/ml).Detection: DAB](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-NB600-250-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-250] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-250-img0012.jpg)
![Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505] - Analysis of extracts of various cell lines, using ASH2L Rabbit mAb (NBP3-16505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Western-Blot-NBP3-16505-img0007.jpg "Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505] - Analysis of extracts of various cell lines, using ASH2L Rabbit mAb (NBP3-16505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded human cervix cancer tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-72202512435315.jpg)
![Immunocytochemistry/ Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Confocal imaging of C2C12 cells using ASH2L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-72202512432043.jpg)
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded mouse brain tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-7220251245375.jpg)
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded rat brain tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-72202512432083.jpg)
![Immunocytochemistry/ Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Confocal imaging of NIH/3T3 cells using ASH2L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-7220251245335.jpg)
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded human colon carcinoma tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-7220251245353.jpg)
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded mouse lung tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-72202512435389.jpg)
![Immunocytochemistry/ Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Confocal imaging of paraffin-embedded Mouse colon tissue using ASH2L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-7220251245345.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0006.jpg "Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of rectum cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Immunohistochemistry-NBP2-61685-img0004.jpg)
![Flow Cytometry: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of K562 cells using ASH2L mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Flow-Cytometry-NBP2-61685-img0002.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0005.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mouse mAb against K562 (1) and F9 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0007.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of colon cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Immunohistochemistry-NBP2-61685-img0003.jpg)
![ELISA: ASH2L Antibody (6F6B9) [NBP2-61685] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-ELISA-NBP2-61685-img0001.jpg)
![Western Blot: ASH2L Antibody [NBP1-88858] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP1-88858-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ASH2L Antibody [NBP1-88858] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ASH2L Antibody [NBP1-88858] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88858-img0008.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody [NBP1-88858] - Staining of human tonsil shows nuclear positivity in non-germinal center.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-Paraffin-NBP1-88858-img0007.jpg)
![Western Blot: ASH2L Antibody [NBP1-88858] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP1-88858-img0006.jpg)
![Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-281-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-281] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-Paraffin-NB600-281-img0013.jpg)
![Chromatin Immunoprecipitation: ASH2L Antibody [NB600-281] - ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-281 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products abeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Chromatin-Immunoprecipitation-NB600-281-img0009.jpg)
![Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Western-Blot-NBP2-61686-img0004.jpg "Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)")
![Immunohistochemistry: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis of paraffin-embedded rectum cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Immunohistochemistry-NBP2-61686-img0003.jpg)
![Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Western-Blot-NBP2-61686-img0005.jpg)
![Immunohistochemistry: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis of paraffin-embedded esophageal cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Immunohistochemistry-NBP2-61686-img0002.jpg)
![ELISA: ASH2L Antibody (6B10H10) [NBP2-61686] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-ELISA-NBP2-61686-img0001.jpg)
![Western Blot: ASH2L Antibody [NBP3-18639] - Figure 1. Western blot analysis using the antibody directed against ASH2L Figure 1A: Western blot was performed using the antibody against hASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on nuclear extracts from HeLa cells (upper panel: lane 1) and ectopically expressed FLAG-tagged ASH2L (ASH2L-F, upper panel: lane 2). The lower panel shows the same western blot with an anti-FLAG antibody. The molecular weight marker is shown on the left.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP3-18639-img0002.jpg "Western Blot: ASH2L Antibody [NBP3-18639] - Figure 1. Western blot analysis using the antibody directed against ASH2L Figure 1A: Western blot was performed using the antibody against hASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk on nuclear extracts from HeLa cells (upper panel: lane 1) and ectopically expressed FLAG-tagged ASH2L (ASH2L-F, upper panel: lane 2). The lower panel shows the same western blot with an anti-FLAG antibody. The molecular weight marker is shown on the left.")
![Western Blot: ASH2L Antibody [NBP3-18639] - Figure 1. Western blot analysis using the antibody directed against ASH2L Figure 1B: Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 ug) with the antibody against ASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/ASH2L Antibody-Western Blot-NBP3-18639-img0001.jpg)
![Western Blot: ASH2L Antibody [NBP2-15488] - Sample (20 ug) A: HeLa nucleus 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP2-15488-img0001.jpg "Western Blot: ASH2L Antibody [NBP2-15488] - Sample (20 ug) A: HeLa nucleus 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: ASH2L Overexpression Lysate (Adult Normal) [NBL1-07765] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASH2L.](http://images.novusbio.com/fullsize/ASH2L-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07765-img0002.jpg "Western Blot: ASH2L Overexpression Lysate (Adult Normal) [NBL1-07765] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ASH2L.")
![Western Blot: ASH2L Antibody [NBP3-18668] - Figure 1. Western blot analysis using the antibody directed against ASH2L Western blot was performed on HeLa nuclear extract (HeLa NE, 20 ug) using the antibody against ASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/ASH2L Antibody-Western Blot-NBP3-18668-img0001.jpg "Western Blot: ASH2L Antibody [NBP3-18668] - Figure 1. Western blot analysis using the antibody directed against ASH2L Western blot was performed on HeLa nuclear extract (HeLa NE, 20 ug) using the antibody against ASH2L diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.")
![Sandwich ELISA: ASH2L Antibody (4G7) [H00009070-M09] - Detection limit for recombinant GST tagged ASH2L is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ASH2L-Antibody-4G7-Sandwich-ELISA-H00009070-M09-img0001.jpg "Sandwich ELISA: ASH2L Antibody (4G7) [H00009070-M09] - Detection limit for recombinant GST tagged ASH2L is 0.1 ng/ml as a capture antibody.")